Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21201 | 5' | -58.7 | NC_004778.3 | + | 121637 | 0.66 | 0.755127 |
Target: 5'- uCGGCCGaGCGCACgUugGUUucGGgGAAGu -3' miRNA: 3'- -GCCGGCaCGCGUG-GugCGA--UCgCUUU- -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 15503 | 0.66 | 0.735678 |
Target: 5'- --aUCGUGCGCcauccGCCGCGCUcgcucuccgcgAGCGAGGa -3' miRNA: 3'- gccGGCACGCG-----UGGUGCGA-----------UCGCUUU- -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 65290 | 0.66 | 0.735678 |
Target: 5'- gCGGCCacggGCGCGaCGCGCUgAGUGAc- -3' miRNA: 3'- -GCCGGca--CGCGUgGUGCGA-UCGCUuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 17045 | 0.66 | 0.734695 |
Target: 5'- aCGuGCCGccuaagcUGCGCgACCGCGCgcuuGCGGu- -3' miRNA: 3'- -GC-CGGC-------ACGCG-UGGUGCGau--CGCUuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 80582 | 0.66 | 0.725812 |
Target: 5'- aGGCCGUGCGCgACCAa-CUGcGCa--- -3' miRNA: 3'- gCCGGCACGCG-UGGUgcGAU-CGcuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 32907 | 0.66 | 0.715866 |
Target: 5'- aCGGCgCGUGCaCGCC-CGCagacGCGAAAc -3' miRNA: 3'- -GCCG-GCACGcGUGGuGCGau--CGCUUU- -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 55989 | 0.66 | 0.705847 |
Target: 5'- uCGGacaGUG-GCGCCGCGUUGGCGu-- -3' miRNA: 3'- -GCCgg-CACgCGUGGUGCGAUCGCuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 52065 | 0.66 | 0.705847 |
Target: 5'- cCGGCgCGUGCGCAUugcaccagCGCGC-GGCGc-- -3' miRNA: 3'- -GCCG-GCACGCGUG--------GUGCGaUCGCuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 65014 | 0.66 | 0.705847 |
Target: 5'- gGGCauucGCGCACCGCGU--GCGAGc -3' miRNA: 3'- gCCGgca-CGCGUGGUGCGauCGCUUu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 53666 | 0.66 | 0.704841 |
Target: 5'- aGGCCGUGUuuGCGCaCAUGCUugccgccAGCGc-- -3' miRNA: 3'- gCCGGCACG--CGUG-GUGCGA-------UCGCuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 112336 | 0.67 | 0.695765 |
Target: 5'- aCGGUag-GCGCGCCGCGCgcacAGCa--- -3' miRNA: 3'- -GCCGgcaCGCGUGGUGCGa---UCGcuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 113778 | 0.67 | 0.68563 |
Target: 5'- uGGUCGUGUuuaauuuguuGCGCCGCGUUugcuugaucaGGCGAu- -3' miRNA: 3'- gCCGGCACG----------CGUGGUGCGA----------UCGCUuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 104149 | 0.67 | 0.675451 |
Target: 5'- gCGGCgacgaaGUGCGCugCGCGUUuugcaaAGUGGAGa -3' miRNA: 3'- -GCCGg-----CACGCGugGUGCGA------UCGCUUU- -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 74155 | 0.67 | 0.675451 |
Target: 5'- uCGGCCGcUGCGC---GCGCcAGCGAc- -3' miRNA: 3'- -GCCGGC-ACGCGuggUGCGaUCGCUuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 17095 | 0.67 | 0.675451 |
Target: 5'- aGGCCGUGuUGCugUGCGCgcGGCGc-- -3' miRNA: 3'- gCCGGCAC-GCGugGUGCGa-UCGCuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 125994 | 0.67 | 0.675451 |
Target: 5'- aGGCC--GCGCAUgACGCUGcGUGGGAg -3' miRNA: 3'- gCCGGcaCGCGUGgUGCGAU-CGCUUU- -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 96583 | 0.67 | 0.675451 |
Target: 5'- gGGUCGUGgaGCACCcUGCUAGCc--- -3' miRNA: 3'- gCCGGCACg-CGUGGuGCGAUCGcuuu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 43305 | 0.67 | 0.67443 |
Target: 5'- aCGGcCCGUGCGCGuugaacuCgGCGCgcauGCGGAc -3' miRNA: 3'- -GCC-GGCACGCGU-------GgUGCGau--CGCUUu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 7822 | 0.67 | 0.665235 |
Target: 5'- uCGGCCGacgGCGUggACCGCGCgcugcAGCaGAAc -3' miRNA: 3'- -GCCGGCa--CGCG--UGGUGCGa----UCG-CUUu -5' |
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21201 | 5' | -58.7 | NC_004778.3 | + | 61915 | 0.67 | 0.654993 |
Target: 5'- aCGGCCG-GCGaauuaAUgGCGUUGGCGGc- -3' miRNA: 3'- -GCCGGCaCGCg----UGgUGCGAUCGCUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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