Results 1 - 20 of 60 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21211 | 5' | -47.6 | NC_004778.3 | + | 17114 | 0.66 | 0.999353 |
Target: 5'- gCGGCGCGCcuaccguggcAAcgccGUUGCCAGCGacacguucUUGGAg -3' miRNA: 3'- gGCUGCGCG----------UU----UAACGGUUGU--------AACUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 79365 | 0.66 | 0.999195 |
Target: 5'- aUCGAuCGCGCGGucugUGCCAAUAa---- -3' miRNA: 3'- -GGCU-GCGCGUUua--ACGGUUGUaacuu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 94626 | 0.66 | 0.999195 |
Target: 5'- -aGugGUGCAAAUgcgcgaUGCCGACAa---- -3' miRNA: 3'- ggCugCGCGUUUA------ACGGUUGUaacuu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 106514 | 0.66 | 0.999195 |
Target: 5'- -aGACGCGCuuuuaGCCAggcuaaauACGUUGAu -3' miRNA: 3'- ggCUGCGCGuuuaaCGGU--------UGUAACUu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 88389 | 0.66 | 0.999195 |
Target: 5'- -gGACGCGCGAc-UGCgAGCggUGGAc -3' miRNA: 3'- ggCUGCGCGUUuaACGgUUGuaACUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 101871 | 0.66 | 0.999195 |
Target: 5'- gUGGCGCGCAGGcagcgGUCcACGUUGGc -3' miRNA: 3'- gGCUGCGCGUUUaa---CGGuUGUAACUu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 27033 | 0.66 | 0.999195 |
Target: 5'- uCCGACGCGUcuAUUG-CGACGggaaUUGGg -3' miRNA: 3'- -GGCUGCGCGuuUAACgGUUGU----AACUu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 45597 | 0.66 | 0.999086 |
Target: 5'- cCUGGCGCGCcuucuGcauagccgcgcccucGUcGCCGGCGUUGAGu -3' miRNA: 3'- -GGCUGCGCGu----U---------------UAaCGGUUGUAACUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 43324 | 0.66 | 0.999006 |
Target: 5'- cUCGGCGCGCA---UGCgGACAa---- -3' miRNA: 3'- -GGCUGCGCGUuuaACGgUUGUaacuu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 61292 | 0.66 | 0.999006 |
Target: 5'- aCGAUGCGCcguc-GCCGACGUgcGAGa -3' miRNA: 3'- gGCUGCGCGuuuaaCGGUUGUAa-CUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 46990 | 0.66 | 0.999006 |
Target: 5'- gCCGACGCGCuug-UGCgAAuCAUcGAc -3' miRNA: 3'- -GGCUGCGCGuuuaACGgUU-GUAaCUu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 58686 | 0.66 | 0.999006 |
Target: 5'- uUGGCGCGCA--UUGCCAAUu----- -3' miRNA: 3'- gGCUGCGCGUuuAACGGUUGuaacuu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 85938 | 0.66 | 0.99878 |
Target: 5'- cCUGACGCaGCGAAUuuUGUCAAUuuguugcgccgGUUGAAc -3' miRNA: 3'- -GGCUGCG-CGUUUA--ACGGUUG-----------UAACUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 2382 | 0.66 | 0.99878 |
Target: 5'- gCCGAaacuucCGCGCGGug-GCCGACGUg--- -3' miRNA: 3'- -GGCU------GCGCGUUuaaCGGUUGUAacuu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 8028 | 0.67 | 0.998625 |
Target: 5'- gCCGACGCGUgccugcugcucagccGcAcgUGCCAGCG-UGAc -3' miRNA: 3'- -GGCUGCGCG---------------U-UuaACGGUUGUaACUu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 10067 | 0.67 | 0.998512 |
Target: 5'- uCCGACGCaGUAAAcugcGCCAGCucacuAUUGAc -3' miRNA: 3'- -GGCUGCG-CGUUUaa--CGGUUG-----UAACUu -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 97403 | 0.67 | 0.998482 |
Target: 5'- gCGGCGCGCAAGgaaGCCGccgguucuaaucuGCAgcGAGu -3' miRNA: 3'- gGCUGCGCGUUUaa-CGGU-------------UGUaaCUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 127886 | 0.67 | 0.998195 |
Target: 5'- aCGGCGCGCAugaacaUGCCGuuguGCAU-GAGc -3' miRNA: 3'- gGCUGCGCGUuua---ACGGU----UGUAaCUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 63328 | 0.67 | 0.998195 |
Target: 5'- uUGcUGUGCGAcUUGUCGGCGUUGAAc -3' miRNA: 3'- gGCuGCGCGUUuAACGGUUGUAACUU- -5' |
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21211 | 5' | -47.6 | NC_004778.3 | + | 100866 | 0.67 | 0.998195 |
Target: 5'- uUGAgGUGCGGAUUGgCCAGC-UUGGc -3' miRNA: 3'- gGCUgCGCGUUUAAC-GGUUGuAACUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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