Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21214 | 5' | -60.1 | NC_004778.3 | + | 4091 | 0.66 | 0.723302 |
Target: 5'- aGAUCGCGCaGCCCgu-ACUuauaCCCAGCg -3' miRNA: 3'- gCUGGUGCGgCGGGaguUGG----GGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 95032 | 0.66 | 0.723302 |
Target: 5'- -cGCCGCGCUGCCggagCAGCCCggCCAc- -3' miRNA: 3'- gcUGGUGCGGCGGga--GUUGGG--GGUug -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 14550 | 0.66 | 0.723302 |
Target: 5'- aCGACCGCG-CGUCCaUGugCgCCAGCa -3' miRNA: 3'- -GCUGGUGCgGCGGGaGUugGgGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 59177 | 0.66 | 0.722331 |
Target: 5'- gCGGCCGCGCaCGCCgUCcaguuuaAAUgCCCAc- -3' miRNA: 3'- -GCUGGUGCG-GCGGgAG-------UUGgGGGUug -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 93721 | 0.66 | 0.713557 |
Target: 5'- uCGACUugGUgCGgCC-CAACCCgCAGCu -3' miRNA: 3'- -GCUGGugCG-GCgGGaGUUGGGgGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 100790 | 0.66 | 0.703745 |
Target: 5'- uCGGCCgcaaaACGgUGCCCUUcg-CCCCGACa -3' miRNA: 3'- -GCUGG-----UGCgGCGGGAGuugGGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 126939 | 0.66 | 0.703745 |
Target: 5'- uCGGCCACGCUGCgCUCGAaugggUgCUUGACa -3' miRNA: 3'- -GCUGGUGCGGCGgGAGUU-----GgGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 91055 | 0.66 | 0.703745 |
Target: 5'- uCGugCACGCCGCC---GACCCgCGu- -3' miRNA: 3'- -GCugGUGCGGCGGgagUUGGGgGUug -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 100091 | 0.66 | 0.703745 |
Target: 5'- gCGugUACaGCCGCUUggcGCaCCCCAGCg -3' miRNA: 3'- -GCugGUG-CGGCGGGaguUG-GGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 64974 | 0.66 | 0.703745 |
Target: 5'- uCGGCauuCGCCGgCCUCGGCgUCgAACg -3' miRNA: 3'- -GCUGgu-GCGGCgGGAGUUGgGGgUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 39107 | 0.66 | 0.693874 |
Target: 5'- uGuuCAUGCCGCgCCUUAAUuacaaCCCGACg -3' miRNA: 3'- gCugGUGCGGCG-GGAGUUGg----GGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 93006 | 0.66 | 0.693874 |
Target: 5'- cCGGCCGCGUgaaCGCgCagCAG-CCCCAACg -3' miRNA: 3'- -GCUGGUGCG---GCGgGa-GUUgGGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 33666 | 0.66 | 0.683954 |
Target: 5'- gCGGCCggcGCGCUGCCgCgcggaUAGCgCCCGGCg -3' miRNA: 3'- -GCUGG---UGCGGCGG-Ga----GUUGgGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 25786 | 0.66 | 0.683954 |
Target: 5'- uGACCGCguuGCCGCCacgCuGCCCCgCcGCg -3' miRNA: 3'- gCUGGUG---CGGCGGga-GuUGGGG-GuUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 33379 | 0.66 | 0.683954 |
Target: 5'- aCGA-CGCGCCGUCUUUGgacGCgCCCAACc -3' miRNA: 3'- -GCUgGUGCGGCGGGAGU---UGgGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 38146 | 0.66 | 0.673993 |
Target: 5'- aGGCCcgcuGCGCCGCUCgacACCUCCAc- -3' miRNA: 3'- gCUGG----UGCGGCGGGaguUGGGGGUug -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 47417 | 0.67 | 0.663999 |
Target: 5'- gGACCACG-CGCCa-----CCCCAACa -3' miRNA: 3'- gCUGGUGCgGCGGgaguugGGGGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 62485 | 0.67 | 0.663999 |
Target: 5'- uCGACCACGCUGUUCUggcgCAGCUCUUuuuGCa -3' miRNA: 3'- -GCUGGUGCGGCGGGA----GUUGGGGGu--UG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 97950 | 0.67 | 0.663999 |
Target: 5'- uGGCCGCGCauuuGCgCUCAGCCaaauaaUCGGCg -3' miRNA: 3'- gCUGGUGCGg---CGgGAGUUGGg-----GGUUG- -5' |
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21214 | 5' | -60.1 | NC_004778.3 | + | 58016 | 0.67 | 0.65398 |
Target: 5'- aCGcCCcCGCCGacaCCCUCGccuacGCCCCCGccgACa -3' miRNA: 3'- -GCuGGuGCGGC---GGGAGU-----UGGGGGU---UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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