Results 1 - 20 of 89 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21216 | 3' | -54.1 | NC_004778.3 | + | 22187 | 0.66 | 0.942617 |
Target: 5'- aACGCCgaaGcCCGCAAAACGUuuaGCa-- -3' miRNA: 3'- gUGCGGa--C-GGCGUUUUGCAug-CGcac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 17934 | 0.66 | 0.942617 |
Target: 5'- gUACGCCUGuuCCGU--GACGgACGUGUa -3' miRNA: 3'- -GUGCGGAC--GGCGuuUUGCaUGCGCAc -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 113147 | 0.66 | 0.937818 |
Target: 5'- gACGCCUGCCGgc--ACGUuuacaacCGCGUc -3' miRNA: 3'- gUGCGGACGGCguuuUGCAu------GCGCAc -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 85316 | 0.66 | 0.937818 |
Target: 5'- cCAUGCCgucGCCGCcgcAAACGccCGCGUc -3' miRNA: 3'- -GUGCGGa--CGGCGu--UUUGCauGCGCAc -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 85286 | 0.66 | 0.937818 |
Target: 5'- cCAUGCCgucGCCGCcgcAAACGccCGCGUc -3' miRNA: 3'- -GUGCGGa--CGGCGu--UUUGCauGCGCAc -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 77371 | 0.66 | 0.937818 |
Target: 5'- cCGCGCgCUGgUGCAAuGCGcACGCGc- -3' miRNA: 3'- -GUGCG-GACgGCGUUuUGCaUGCGCac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 25468 | 0.66 | 0.937818 |
Target: 5'- uGCGCCuUGCCGguuugacaagaCGAAGCuuGUGCGCuGUGu -3' miRNA: 3'- gUGCGG-ACGGC-----------GUUUUG--CAUGCG-CAC- -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 125723 | 0.66 | 0.937818 |
Target: 5'- gCACGCCgaaaUGCCGC---GCGUugGUa-- -3' miRNA: 3'- -GUGCGG----ACGGCGuuuUGCAugCGcac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 38232 | 0.66 | 0.937324 |
Target: 5'- -gUGCCUcUUGCAAAAacguuccaauaucCGUGCGCGUGa -3' miRNA: 3'- guGCGGAcGGCGUUUU-------------GCAUGCGCAC- -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 125598 | 0.66 | 0.932769 |
Target: 5'- uGCGCCgugUGUUaCGAAACGUACGUGa- -3' miRNA: 3'- gUGCGG---ACGGcGUUUUGCAUGCGCac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 20923 | 0.66 | 0.932769 |
Target: 5'- aUACGCCaugGCCGUcucGAACG-ACGCGc- -3' miRNA: 3'- -GUGCGGa--CGGCGu--UUUGCaUGCGCac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 98721 | 0.66 | 0.927469 |
Target: 5'- gGCaGCCgGCCGCGGccgcAGCGUGCuuCGUGu -3' miRNA: 3'- gUG-CGGaCGGCGUU----UUGCAUGc-GCAC- -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 114649 | 0.66 | 0.927469 |
Target: 5'- -uUGCa-GCCGCGAAGCGUuuGCGCa-- -3' miRNA: 3'- guGCGgaCGGCGUUUUGCA--UGCGcac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 58270 | 0.66 | 0.927469 |
Target: 5'- aAUGCUcGCCGCGcuucAAGCGcUGCuGCGUGu -3' miRNA: 3'- gUGCGGaCGGCGU----UUUGC-AUG-CGCAC- -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 53681 | 0.66 | 0.927469 |
Target: 5'- aCAUGCUUGCCGCc-AGCGccgaggaGCGCGa- -3' miRNA: 3'- -GUGCGGACGGCGuuUUGCa------UGCGCac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 90251 | 0.66 | 0.927469 |
Target: 5'- aGCGCCUGCaugacCGUAGcgGGCG-ACGCGgUGg -3' miRNA: 3'- gUGCGGACG-----GCGUU--UUGCaUGCGC-AC- -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 86646 | 0.66 | 0.927469 |
Target: 5'- -cCGCC-GCCGaCAAcGCGUGCGCc-- -3' miRNA: 3'- guGCGGaCGGC-GUUuUGCAUGCGcac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 55140 | 0.66 | 0.927469 |
Target: 5'- aGCGCgaGCCGCAGAcCGgcCuCGUGc -3' miRNA: 3'- gUGCGgaCGGCGUUUuGCauGcGCAC- -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 69610 | 0.66 | 0.92135 |
Target: 5'- gACGaCCUGCC-CAgucauaaagcuaaGAGCGUGCGCa-- -3' miRNA: 3'- gUGC-GGACGGcGU-------------UUUGCAUGCGcac -5' |
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21216 | 3' | -54.1 | NC_004778.3 | + | 88692 | 0.67 | 0.916117 |
Target: 5'- gAUGUC-GCCGCGcagcAAGCG-GCGCGUGc -3' miRNA: 3'- gUGCGGaCGGCGU----UUUGCaUGCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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