Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22564 | 3' | -54.3 | NC_005091.1 | + | 19405 | 0.66 | 0.781683 |
Target: 5'- aAGCcgGCAUGugcgaGCGCGGCGCu--CGGg -3' miRNA: 3'- -UUGa-CGUACug---UGCGUCGCGcauGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 51628 | 0.66 | 0.771565 |
Target: 5'- ---cGCAUGGC-CGCGuaucagauacuGCGCGgggGCGGu -3' miRNA: 3'- uugaCGUACUGuGCGU-----------CGCGCa--UGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 49439 | 0.66 | 0.771565 |
Target: 5'- uGACUGCAuccuuagacguUGaACACGUAGCGC--ACGa -3' miRNA: 3'- -UUGACGU-----------AC-UGUGCGUCGCGcaUGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 35571 | 0.66 | 0.767476 |
Target: 5'- uGGCUGCcUGACagcuacuACGUccagcucgucuccgAGCgGCGUACGGa -3' miRNA: 3'- -UUGACGuACUG-------UGCG--------------UCG-CGCAUGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 35084 | 0.66 | 0.7613 |
Target: 5'- --aUGCgGUGAC-UGCGGCcCGUGCGGc -3' miRNA: 3'- uugACG-UACUGuGCGUCGcGCAUGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 25046 | 0.66 | 0.7613 |
Target: 5'- aGACUGCGaGACugGuCGGCacuGCG-GCGGc -3' miRNA: 3'- -UUGACGUaCUGugC-GUCG---CGCaUGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 29960 | 0.66 | 0.7509 |
Target: 5'- cACUcgGCAUcACAuUGCAGCGCG-ACGGc -3' miRNA: 3'- uUGA--CGUAcUGU-GCGUCGCGCaUGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 11332 | 0.66 | 0.740376 |
Target: 5'- cGGCUGCcgcguucGACuGCGCAuGUGCGUcACGGa -3' miRNA: 3'- -UUGACGua-----CUG-UGCGU-CGCGCA-UGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 766 | 0.67 | 0.729741 |
Target: 5'- cACUGCGgauuCGCGCGGCGCaGUccGCGu -3' miRNA: 3'- uUGACGUacu-GUGCGUCGCG-CA--UGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 57405 | 0.67 | 0.719006 |
Target: 5'- gAAUUGCGcGGuCAUGCAGCGCaUGCGc -3' miRNA: 3'- -UUGACGUaCU-GUGCGUCGCGcAUGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 11 | 0.67 | 0.719006 |
Target: 5'- gAAUUGCGcGGuCAUGCAGCGCaUGCGc -3' miRNA: 3'- -UUGACGUaCU-GUGCGUCGCGcAUGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 52115 | 0.67 | 0.708184 |
Target: 5'- aGGCgGCcgucgccGGCGCGgGGCGCGUuGCGGa -3' miRNA: 3'- -UUGaCGua-----CUGUGCgUCGCGCA-UGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 49722 | 0.67 | 0.708184 |
Target: 5'- -uCUGCAUGcgggGCACGuCGGC-CGUGCGu -3' miRNA: 3'- uuGACGUAC----UGUGC-GUCGcGCAUGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 19128 | 0.67 | 0.708184 |
Target: 5'- uACUGCGUgGGCGgGCAGCGUcucgacuaGCGGu -3' miRNA: 3'- uUGACGUA-CUGUgCGUCGCGca------UGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 26146 | 0.67 | 0.708184 |
Target: 5'- cGCUGuCGUGGUAU-CAGCGCGUGCGcGg -3' miRNA: 3'- uUGAC-GUACUGUGcGUCGCGCAUGC-C- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 40016 | 0.67 | 0.686322 |
Target: 5'- gGAgUGCAUGAC-CGCgaAGCGCaagugGUACGa -3' miRNA: 3'- -UUgACGUACUGuGCG--UCGCG-----CAUGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 39681 | 0.67 | 0.686322 |
Target: 5'- --aUGCGUcGACACG--GCGCGUACGu -3' miRNA: 3'- uugACGUA-CUGUGCguCGCGCAUGCc -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 51587 | 0.67 | 0.675306 |
Target: 5'- ---cGCGUGACggGCGCgcagaAGCGCGgcACGGg -3' miRNA: 3'- uugaCGUACUG--UGCG-----UCGCGCa-UGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 9635 | 0.67 | 0.675306 |
Target: 5'- uGGCUGCc-GACACgGCAGCcucgucacGgGUGCGGg -3' miRNA: 3'- -UUGACGuaCUGUG-CGUCG--------CgCAUGCC- -5' |
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22564 | 3' | -54.3 | NC_005091.1 | + | 41730 | 0.67 | 0.675306 |
Target: 5'- aAACUgGCGcuGCACGaUGGCGCGUGCGu -3' miRNA: 3'- -UUGA-CGUacUGUGC-GUCGCGCAUGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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