Results 1 - 20 of 82 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22574 | 5' | -60.1 | NC_005091.1 | + | 40858 | 0.66 | 0.526198 |
Target: 5'- -cCCCGCuCGGauggcguucgugACCGUgcagaGCGCCCaGUCGUu -3' miRNA: 3'- caGGGCG-GCU------------UGGCG-----UGUGGG-CAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 16659 | 0.66 | 0.526198 |
Target: 5'- -gCCCGUgGAGCCGCugaaagcggucCACCCGa-GCu -3' miRNA: 3'- caGGGCGgCUUGGCGu----------GUGGGCagCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 22765 | 0.66 | 0.526198 |
Target: 5'- -aCCCGCUGAcggccuCUGCGCAggucggagauaCCGUCGUg -3' miRNA: 3'- caGGGCGGCUu-----GGCGUGUg----------GGCAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 40872 | 0.66 | 0.526198 |
Target: 5'- gGUCUCGCCGGAUgCGUGCGCag--CGCg -3' miRNA: 3'- -CAGGGCGGCUUG-GCGUGUGggcaGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 14926 | 0.66 | 0.526198 |
Target: 5'- cUCCCGCCuguCauggGCGCAUCCGgugguUCGCa -3' miRNA: 3'- cAGGGCGGcuuGg---CGUGUGGGC-----AGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 15046 | 0.66 | 0.526198 |
Target: 5'- cUCCCGCCuguCauggGCGCAUCCGgugguUCGCa -3' miRNA: 3'- cAGGGCGGcuuGg---CGUGUGGGC-----AGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 15166 | 0.66 | 0.526198 |
Target: 5'- cUCCCGCCuguCauggGCGCAUCCGgugguUCGCa -3' miRNA: 3'- cAGGGCGGcuuGg---CGUGUGGGC-----AGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 13503 | 0.66 | 0.520139 |
Target: 5'- --gCCGCCGAGaCCGUucacgucgcugcucgACGCCgGgUCGCu -3' miRNA: 3'- cagGGCGGCUU-GGCG---------------UGUGGgC-AGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 51043 | 0.66 | 0.516116 |
Target: 5'- -gCuuGCCGAuuugcCCGUGCGCaCCG-CGCu -3' miRNA: 3'- caGggCGGCUu----GGCGUGUG-GGCaGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 56362 | 0.66 | 0.516116 |
Target: 5'- cGUCCCGCCaGaAGCCcCAUACgCCaGcCGCa -3' miRNA: 3'- -CAGGGCGG-C-UUGGcGUGUG-GG-CaGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 20399 | 0.66 | 0.516116 |
Target: 5'- aUCCCGUgGAACaacaGUACGuaCGUUGCg -3' miRNA: 3'- cAGGGCGgCUUGg---CGUGUggGCAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 7476 | 0.66 | 0.506112 |
Target: 5'- -aCCaaGCCGAGgCGCACcagACCaGUCGCu -3' miRNA: 3'- caGGg-CGGCUUgGCGUG---UGGgCAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 31318 | 0.66 | 0.506112 |
Target: 5'- aUCaacCCGAACCGUACGCUcaagCGUCGUg -3' miRNA: 3'- cAGggcGGCUUGGCGUGUGG----GCAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 40620 | 0.66 | 0.506112 |
Target: 5'- cUUCCGgguacUCGAcGCCGUACAgCCgCGUCGCg -3' miRNA: 3'- cAGGGC-----GGCU-UGGCGUGU-GG-GCAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 9299 | 0.66 | 0.496193 |
Target: 5'- --aCCGCCGGaaacACCGCGCgAUUCGggUCGCc -3' miRNA: 3'- cagGGCGGCU----UGGCGUG-UGGGC--AGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 12590 | 0.66 | 0.496193 |
Target: 5'- --gUgGCCGAuUCGCGCGCCCGgucucggaugUCGCc -3' miRNA: 3'- cagGgCGGCUuGGCGUGUGGGC----------AGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 12978 | 0.66 | 0.496193 |
Target: 5'- cGUCCgGgCCGAGCgGCGuauUGCCUGcCGCc -3' miRNA: 3'- -CAGGgC-GGCUUGgCGU---GUGGGCaGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 37137 | 0.66 | 0.49225 |
Target: 5'- gGUCUCGuuGAACCaaucgcccacguaCGCGCCCGgCGUg -3' miRNA: 3'- -CAGGGCggCUUGGc------------GUGUGGGCaGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 17634 | 0.66 | 0.486363 |
Target: 5'- gGUUgCGUCGAcacgcucccgGCCGCGCAUCU-UCGCa -3' miRNA: 3'- -CAGgGCGGCU----------UGGCGUGUGGGcAGCG- -5' |
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22574 | 5' | -60.1 | NC_005091.1 | + | 31409 | 0.66 | 0.486363 |
Target: 5'- cGUCgCGCCGAACgUGCAagGCCgCGugaUCGCu -3' miRNA: 3'- -CAGgGCGGCUUG-GCGUg-UGG-GC---AGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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