Results 1 - 20 of 51 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22586 | 3' | -53.4 | NC_005091.1 | + | 56881 | 0.66 | 0.841542 |
Target: 5'- gCGGUGCAGCCaCGCcaUCGcgUUGCccGCg -3' miRNA: 3'- -GUCGCGUUGGaGCGa-AGCuaAGCG--CG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 3998 | 0.66 | 0.841542 |
Target: 5'- aAGCGCGaauGCCUCGCgccgaucucUCGAUuUCGgaaGCc -3' miRNA: 3'- gUCGCGU---UGGAGCGa--------AGCUA-AGCg--CG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 38541 | 0.66 | 0.841542 |
Target: 5'- uCAGCGCGGCUgcgguuucUGCUUCGAUcUCG-GUu -3' miRNA: 3'- -GUCGCGUUGGa-------GCGAAGCUA-AGCgCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 52033 | 0.66 | 0.840659 |
Target: 5'- gAGCGUGACUuccucaaUCGCga-GAUaCGCGCc -3' miRNA: 3'- gUCGCGUUGG-------AGCGaagCUAaGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 3016 | 0.66 | 0.836216 |
Target: 5'- -cGCGUuGCCUCucgauagcccgcagaGCgagaacgCGAUUCGCGUg -3' miRNA: 3'- guCGCGuUGGAG---------------CGaa-----GCUAAGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 23858 | 0.66 | 0.832621 |
Target: 5'- aCGGagaGCGGCC-CGCa--GAUUCaGCGCa -3' miRNA: 3'- -GUCg--CGUUGGaGCGaagCUAAG-CGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 6525 | 0.66 | 0.832621 |
Target: 5'- uCAGCGCAGCgCgcaCGCUgcccUCGGga-GCGUc -3' miRNA: 3'- -GUCGCGUUG-Ga--GCGA----AGCUaagCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 25723 | 0.66 | 0.832621 |
Target: 5'- -uGCGCGGCCa-GCUcggcgaUCGGcugcccgccgcaUUCGCGCu -3' miRNA: 3'- guCGCGUUGGagCGA------AGCU------------AAGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 51679 | 0.66 | 0.823486 |
Target: 5'- -cGCGCccguGCCgCGCUUCug--CGCGCc -3' miRNA: 3'- guCGCGu---UGGaGCGAAGcuaaGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 4660 | 0.66 | 0.823486 |
Target: 5'- -cGCGCAGCUcCGCgcgUCGuUUUGgGCc -3' miRNA: 3'- guCGCGUUGGaGCGa--AGCuAAGCgCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 36624 | 0.66 | 0.823486 |
Target: 5'- cCGGgGCGACCgUCGCcgUCGAUacuggaaGUGCc -3' miRNA: 3'- -GUCgCGUUGG-AGCGa-AGCUAag-----CGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 29563 | 0.66 | 0.823486 |
Target: 5'- cCAGCGUccuACgUCGCUcgccCGGUUCGC-Cg -3' miRNA: 3'- -GUCGCGu--UGgAGCGAa---GCUAAGCGcG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 45999 | 0.66 | 0.814146 |
Target: 5'- gCGGCGCAACaccggcaaCUCggauGCUUCGAUgUCGaGCg -3' miRNA: 3'- -GUCGCGUUG--------GAG----CGAAGCUA-AGCgCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 448 | 0.66 | 0.814146 |
Target: 5'- gAGCaGCAAUCg-GCggUCGAUcagCGCGCa -3' miRNA: 3'- gUCG-CGUUGGagCGa-AGCUAa--GCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 57842 | 0.66 | 0.814146 |
Target: 5'- gAGCaGCAAUCg-GCggUCGAUcagCGCGCa -3' miRNA: 3'- gUCG-CGUUGGagCGa-AGCUAa--GCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 7893 | 0.66 | 0.804612 |
Target: 5'- -uGuCGUAGCCUCGCgaCGGUUCGaUGg -3' miRNA: 3'- guC-GCGUUGGAGCGaaGCUAAGC-GCg -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 33552 | 0.66 | 0.803648 |
Target: 5'- gAGCGCGaaaucgcgcGCCUCGgcggagaCUUCcGAaagagcUUCGCGCa -3' miRNA: 3'- gUCGCGU---------UGGAGC-------GAAG-CU------AAGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 5086 | 0.67 | 0.785003 |
Target: 5'- gCGGCGCAGga-CGCgaUCGAaugcUUCGCGCu -3' miRNA: 3'- -GUCGCGUUggaGCGa-AGCU----AAGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 33423 | 0.67 | 0.785003 |
Target: 5'- gCAGgGCGuCCUCGCgagCGcgcUGCGCg -3' miRNA: 3'- -GUCgCGUuGGAGCGaa-GCuaaGCGCG- -5' |
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22586 | 3' | -53.4 | NC_005091.1 | + | 11386 | 0.67 | 0.785003 |
Target: 5'- gGGCGuCGGCCgcgucacgcugCGCUUCGGccugccccuguUUCGCGg -3' miRNA: 3'- gUCGC-GUUGGa----------GCGAAGCU-----------AAGCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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