Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22586 | 5' | -57.7 | NC_005091.1 | + | 48640 | 0.66 | 0.580052 |
Target: 5'- uGGCGCgcaGGcCCGAUGAggaucgcuCGGGGCGGCc -3' miRNA: 3'- uUCGUGa--CC-GGCUGUU--------GUUCCGCCGc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 810 | 0.66 | 0.580052 |
Target: 5'- -uGCGCUGcGCUGccGCGugGGuguacGGCGGCGa -3' miRNA: 3'- uuCGUGAC-CGGC--UGUugUU-----CCGCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 55371 | 0.66 | 0.573543 |
Target: 5'- -uGCcgACUGGauGGCAACGAGGCgaccaaggaagucauGGCGg -3' miRNA: 3'- uuCG--UGACCggCUGUUGUUCCG---------------CCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 16820 | 0.66 | 0.56813 |
Target: 5'- gGAGCGugGGCCaaucacgggGACGACGuagcggccuuccuAGGCGGCGc -3' miRNA: 3'- -UUCGUgaCCGG---------CUGUUGU-------------UCCGCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 30159 | 0.66 | 0.558423 |
Target: 5'- aAAGCGCUGGuCCGuCAgacuguccaugACAcucuGGCGGUa -3' miRNA: 3'- -UUCGUGACC-GGCuGU-----------UGUu---CCGCCGc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 36439 | 0.66 | 0.547692 |
Target: 5'- gGGGCGuCUGGCUgaagGACGGCGAGucgaugaCGGCGg -3' miRNA: 3'- -UUCGU-GACCGG----CUGUUGUUCc------GCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 52087 | 0.66 | 0.537027 |
Target: 5'- -cGCAUUGGCgCGGCcguauGCcgugaaAAGGCGGCc -3' miRNA: 3'- uuCGUGACCG-GCUGu----UG------UUCCGCCGc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 4225 | 0.66 | 0.537027 |
Target: 5'- cGGCugUGGCaGAgGAUgAAGuGCGGCGu -3' miRNA: 3'- uUCGugACCGgCUgUUG-UUC-CGCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 40636 | 0.66 | 0.526435 |
Target: 5'- cGGCGCgGGauaCCGACu-CGGcGGCGGCGa -3' miRNA: 3'- uUCGUGaCC---GGCUGuuGUU-CCGCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 31781 | 0.67 | 0.515922 |
Target: 5'- cGGCACgagcaGGCCGcCGACGAGcGCGaCGa -3' miRNA: 3'- uUCGUGa----CCGGCuGUUGUUC-CGCcGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 48818 | 0.67 | 0.515922 |
Target: 5'- -cGCGCUgcgccguacGGCCGAUGACAu-GCGGCc -3' miRNA: 3'- uuCGUGA---------CCGGCUGUUGUucCGCCGc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 22178 | 0.67 | 0.505495 |
Target: 5'- -cGCGCaGGUCGGCAuuuuGCu-GGCGGCc -3' miRNA: 3'- uuCGUGaCCGGCUGU----UGuuCCGCCGc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 28379 | 0.67 | 0.49516 |
Target: 5'- -cGCuCUGGuucaCCGGCAGCAAGGCGa-- -3' miRNA: 3'- uuCGuGACC----GGCUGUUGUUCCGCcgc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 10341 | 0.67 | 0.484921 |
Target: 5'- aGAGUACUGcGCgugucgcgcgccCGACAuCGAGGaCGGCGc -3' miRNA: 3'- -UUCGUGAC-CG------------GCUGUuGUUCC-GCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 52383 | 0.67 | 0.477815 |
Target: 5'- cAGCACUGGCCGcugucgaaucgauccGCAACGcacacuaAGG-GGCu -3' miRNA: 3'- uUCGUGACCGGC---------------UGUUGU-------UCCgCCGc -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 55099 | 0.67 | 0.474785 |
Target: 5'- uAAGCACgaggGGUCGuCAGCAcGGGauacCGGCGg -3' miRNA: 3'- -UUCGUGa---CCGGCuGUUGU-UCC----GCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 4822 | 0.67 | 0.474785 |
Target: 5'- -cGCGCUcGGCgCGGCGGCAAcGGUcaagcgGGCGa -3' miRNA: 3'- uuCGUGA-CCG-GCUGUUGUU-CCG------CCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 31093 | 0.67 | 0.474785 |
Target: 5'- cGGCGCUcaCCGuGCAACugacGGGCGGCGc -3' miRNA: 3'- uUCGUGAccGGC-UGUUGu---UCCGCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 27540 | 0.67 | 0.464756 |
Target: 5'- gAAGCACUGGCCGAUGuguuuGCAAcGGagcugucuuucuCGGUGc -3' miRNA: 3'- -UUCGUGACCGGCUGU-----UGUU-CC------------GCCGC- -5' |
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22586 | 5' | -57.7 | NC_005091.1 | + | 17336 | 0.67 | 0.464756 |
Target: 5'- uAGCGCUGGCCGuuCAGCuucAGauugccCGGCGg -3' miRNA: 3'- uUCGUGACCGGCu-GUUGu--UCc-----GCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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