Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22914 | 5' | -49.2 | NC_005137.2 | + | 1690 | 0.72 | 0.870216 |
Target: 5'- cAACGCGCGCAAUcUUAGUcCCGAc-- -3' miRNA: 3'- -UUGCGCGUGUUAaAAUCGcGGCUacu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 98365 | 0.66 | 0.995822 |
Target: 5'- -uUGUGCGCcggUUUGGCGCCGucgcGAc -3' miRNA: 3'- uuGCGCGUGuuaAAAUCGCGGCua--CU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 2473 | 0.66 | 0.995822 |
Target: 5'- -uCGCGCGCuAUUUauGCGCCGcGUGc -3' miRNA: 3'- uuGCGCGUGuUAAAauCGCGGC-UACu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 128665 | 0.66 | 0.99509 |
Target: 5'- gAACGCGCGUAAUUgu-GaCGCCGAa-- -3' miRNA: 3'- -UUGCGCGUGUUAAaauC-GCGGCUacu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 44037 | 0.66 | 0.993314 |
Target: 5'- uGCGCGCACGGUUUUGccuGUGCUa---- -3' miRNA: 3'- uUGCGCGUGUUAAAAU---CGCGGcuacu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 130950 | 0.67 | 0.991908 |
Target: 5'- cGACGCGCGCGcUUUUAuacaagcgguugacGCGcCCGAaGAc -3' miRNA: 3'- -UUGCGCGUGUuAAAAU--------------CGC-GGCUaCU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 117190 | 0.67 | 0.991058 |
Target: 5'- uACGaCGCACAAUUUcc-CGCCGAUu- -3' miRNA: 3'- uUGC-GCGUGUUAAAaucGCGGCUAcu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 90505 | 0.67 | 0.990276 |
Target: 5'- aAGCGgGCACAAUggaccaugauuuggUUAGCGuuGgcGAg -3' miRNA: 3'- -UUGCgCGUGUUAa-------------AAUCGCggCuaCU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 61542 | 0.67 | 0.989725 |
Target: 5'- cGCGCGCGCGAUUUUuuuCGuuGAg-- -3' miRNA: 3'- uUGCGCGUGUUAAAAuc-GCggCUacu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 54746 | 0.67 | 0.989725 |
Target: 5'- uGGCGCGCACAAc-----CGCCGAaaUGGa -3' miRNA: 3'- -UUGCGCGUGUUaaaaucGCGGCU--ACU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 60512 | 0.67 | 0.988242 |
Target: 5'- cACGUGCucaAAUUgUGGCGCUGAUuGAa -3' miRNA: 3'- uUGCGCGug-UUAAaAUCGCGGCUA-CU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 93681 | 0.68 | 0.978206 |
Target: 5'- aGAUGCGCaACAGgcccAGCGCCGGa-- -3' miRNA: 3'- -UUGCGCG-UGUUaaaaUCGCGGCUacu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 123105 | 0.69 | 0.973078 |
Target: 5'- aAAUGCGCAauggagcuGCGCCGGUGu -3' miRNA: 3'- -UUGCGCGUguuaaaauCGCGGCUACu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 124933 | 0.69 | 0.971896 |
Target: 5'- uGAUGUGCuugaucgccggguuGCGAUgaacgcGGCGCCGGUGAu -3' miRNA: 3'- -UUGCGCG--------------UGUUAaaa---UCGCGGCUACU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 28520 | 0.7 | 0.955108 |
Target: 5'- uGACGCGC---GUUUcGGCGuuGAUGAc -3' miRNA: 3'- -UUGCGCGuguUAAAaUCGCggCUACU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 41822 | 0.79 | 0.543528 |
Target: 5'- cGGCGCGcCACGcgUuugUUAGCGCUGGUGAg -3' miRNA: 3'- -UUGCGC-GUGUuaA---AAUCGCGGCUACU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 45609 | 0.66 | 0.996462 |
Target: 5'- aAACGCGUGCGcgUgcuGCGCCGc--- -3' miRNA: 3'- -UUGCGCGUGUuaAaauCGCGGCuacu -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 19691 | 0.66 | 0.995822 |
Target: 5'- cGGCGgGCACAgaacgccuucGUUUgccUGGCGCCGuuuccguUGAa -3' miRNA: 3'- -UUGCgCGUGU----------UAAA---AUCGCGGCu------ACU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 2627 | 0.66 | 0.993314 |
Target: 5'- cACGCgGCGCAuaaaUAGCGCgCGAUaGAg -3' miRNA: 3'- uUGCG-CGUGUuaaaAUCGCG-GCUA-CU- -5' |
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22914 | 5' | -49.2 | NC_005137.2 | + | 128863 | 0.67 | 0.991552 |
Target: 5'- gGGCGUGCACGAcggcacguuggaauaUUUUAGCcCCGAa-- -3' miRNA: 3'- -UUGCGCGUGUU---------------AAAAUCGcGGCUacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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