Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23034 | 3' | -58.1 | NC_005178.1 | + | 34910 | 0.67 | 0.381487 |
|
Target: 5'- cGGUCGGGCGgaau-CCAGCcCGGUGg -3' miRNA: 3'- -CCGGCUCGCgaaguGGUCGaGCCACg -5' |
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| 23034 | 3' | -58.1 | NC_005178.1 | + | 35172 | 0.66 | 0.437659 |
|
Target: 5'- cGGaCCGGcCGCcagCGgCAGCUCGGcgGCg -3' miRNA: 3'- -CC-GGCUcGCGaa-GUgGUCGAGCCa-CG- -5' |
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| 23034 | 3' | -58.1 | NC_005178.1 | + | 36011 | 0.7 | 0.256698 |
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Target: 5'- aGGCCGAGgGCgacacuuacauuUUCACCggGGC-CGGgGCg -3' miRNA: 3'- -CCGGCUCgCG------------AAGUGG--UCGaGCCaCG- -5' |
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| 23034 | 3' | -58.1 | NC_005178.1 | + | 36371 | 0.67 | 0.372603 |
|
Target: 5'- cGCUGAuucuGCGCUUCACCGGgUCGuuuuccaGCa -3' miRNA: 3'- cCGGCU----CGCGAAGUGGUCgAGCca-----CG- -5' |
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| 23034 | 3' | -58.1 | NC_005178.1 | + | 36535 | 0.67 | 0.390511 |
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Target: 5'- cGGCCGAGCGgUagccUUGCCcGCUaCGGgGUg -3' miRNA: 3'- -CCGGCUCGCgA----AGUGGuCGA-GCCaCG- -5' |
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| 23034 | 3' | -58.1 | NC_005178.1 | + | 37236 | 0.7 | 0.27727 |
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Target: 5'- cGGCCuccaGGCGCagCGggUCGGaCUCGGUGCg -3' miRNA: 3'- -CCGGc---UCGCGaaGU--GGUC-GAGCCACG- -5' |
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| 23034 | 3' | -58.1 | NC_005178.1 | + | 37558 | 0.69 | 0.284414 |
|
Target: 5'- cGGCCGAGCuGCUcgaugcauucgCGCUGGUccUCGGUGa -3' miRNA: 3'- -CCGGCUCG-CGAa----------GUGGUCG--AGCCACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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