Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23142 | 3' | -65.7 | NC_005178.1 | + | 37492 | 0.66 | 0.161714 |
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Target: 5'- cCAGCGCGAaugcaucgaGCAGCUCGGcCGaUUCCUg -3' miRNA: 3'- uGUCGCGCU---------CGUCGGGCC-GC-GGGGAg -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 28815 | 0.66 | 0.170077 |
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Target: 5'- cACGGCaaggGCGaAGUAGCCCucgccugGGUGUUCCUCc -3' miRNA: 3'- -UGUCG----CGC-UCGUCGGG-------CCGCGGGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 31243 | 0.66 | 0.170528 |
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Target: 5'- gACAGCaucguCGAGC-GCCUGaGCGCCUCg- -3' miRNA: 3'- -UGUCGc----GCUCGuCGGGC-CGCGGGGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 21944 | 0.66 | 0.170528 |
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Target: 5'- aACAGCuCG-GCcuAGCCUggaGGUGCCCCUg -3' miRNA: 3'- -UGUCGcGCuCG--UCGGG---CCGCGGGGAg -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 20751 | 0.66 | 0.179769 |
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Target: 5'- aACAGCa-GAGC-GCCgGGCGUaCCUCg -3' miRNA: 3'- -UGUCGcgCUCGuCGGgCCGCGgGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 9394 | 0.66 | 0.179769 |
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Target: 5'- uACAGC-CGAGgcgccgaccguCAGCCCcugGGCGCCgCCaUCg -3' miRNA: 3'- -UGUCGcGCUC-----------GUCGGG---CCGCGG-GG-AG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 18138 | 0.66 | 0.179769 |
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Target: 5'- -aGGC-CGAGCAGCUCGGCugcGCgCCg- -3' miRNA: 3'- ugUCGcGCUCGUCGGGCCG---CGgGGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 33016 | 0.66 | 0.179769 |
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Target: 5'- gGCGGCGUccggcucgauGGGCAGgCCGGagcguUGCCgCUCg -3' miRNA: 3'- -UGUCGCG----------CUCGUCgGGCC-----GCGGgGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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