Results 1 - 20 of 187 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23220 | 5' | -61.6 | NC_005259.1 | + | 35861 | 0.66 | 0.502281 |
Target: 5'- uCCGccGCCGcGUCcaccgcaGCCGCCGCCGa -3' miRNA: 3'- cGGCucCGGC-UAGag-----CGGUGGCGGCg -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 4895 | 0.66 | 0.502281 |
Target: 5'- aCCGAGGgUGAUCggaugaGCUGCUuuGCCGUa -3' miRNA: 3'- cGGCUCCgGCUAGag----CGGUGG--CGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 44512 | 0.66 | 0.502281 |
Target: 5'- uGCCcuGGCCacccaCGCCGCCGCCcgGCa -3' miRNA: 3'- -CGGcuCCGGcuagaGCGGUGGCGG--CG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 15837 | 0.66 | 0.502281 |
Target: 5'- aGCuCGAuguacGGCuCGGgcaUCGCCAagcUCGCCGCu -3' miRNA: 3'- -CG-GCU-----CCG-GCUag-AGCGGU---GGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 56770 | 0.66 | 0.502281 |
Target: 5'- aUCGGGGaCCG--CUUGaCACCGCCGUu -3' miRNA: 3'- cGGCUCC-GGCuaGAGCgGUGGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 14371 | 0.66 | 0.502281 |
Target: 5'- uGUCuccGCUGAUCUCGUCACCGagcuCGCc -3' miRNA: 3'- -CGGcucCGGCUAGAGCGGUGGCg---GCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 67108 | 0.66 | 0.50131 |
Target: 5'- uCCGAGGUguacugcccgcugCGGUCgugCGUgagCACCGCUGUg -3' miRNA: 3'- cGGCUCCG-------------GCUAGa--GCG---GUGGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 17846 | 0.66 | 0.499371 |
Target: 5'- gGCCGAGGCCaccccguggucagcGAUCcuugaGCCGCuCGguuggacccCCGCa -3' miRNA: 3'- -CGGCUCCGG--------------CUAGag---CGGUG-GC---------GGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 33599 | 0.66 | 0.497435 |
Target: 5'- aCCGAGGCgCGGUCgagcugcaaccgGCCACCGaaacuguugcgaaUCGCg -3' miRNA: 3'- cGGCUCCG-GCUAGag----------CGGUGGC-------------GGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 16622 | 0.66 | 0.492611 |
Target: 5'- -aCG-GGCCGAggCagGCCACCuacggcgacGCCGCc -3' miRNA: 3'- cgGCuCCGGCUa-GagCGGUGG---------CGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 33133 | 0.66 | 0.492611 |
Target: 5'- aGCCGccGCgcgUGAUCUUGCCguagACCGCCu- -3' miRNA: 3'- -CGGCucCG---GCUAGAGCGG----UGGCGGcg -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 17772 | 0.66 | 0.492611 |
Target: 5'- cGCCG-GGCCGAg--CGC-ACCGagcgugcgcaCCGCg -3' miRNA: 3'- -CGGCuCCGGCUagaGCGgUGGC----------GGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 68745 | 0.66 | 0.492611 |
Target: 5'- uGCCGAGcucgaccccGgCGAcCUCGCCAUCGgUGUa -3' miRNA: 3'- -CGGCUC---------CgGCUaGAGCGGUGGCgGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 14668 | 0.66 | 0.491649 |
Target: 5'- cCCGAcucgGGCCGcGUgcacucgauucugCUCGaCCcCCGCCGCg -3' miRNA: 3'- cGGCU----CCGGC-UA-------------GAGC-GGuGGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 20701 | 0.66 | 0.483027 |
Target: 5'- cGCuCGGuGGUCGAaggUUUCaCC-CCGCCGCg -3' miRNA: 3'- -CG-GCU-CCGGCU---AGAGcGGuGGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 5494 | 0.66 | 0.483027 |
Target: 5'- cGCCGAGcGagGAUCgagCuaCGCgCGCCGCg -3' miRNA: 3'- -CGGCUC-CggCUAGa--GcgGUG-GCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 66020 | 0.66 | 0.477321 |
Target: 5'- cUCGGGGCCgcacguguugcgcgcGAgCUCGacguagaCGCCGCCGUg -3' miRNA: 3'- cGGCUCCGG---------------CUaGAGCg------GUGGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 38517 | 0.66 | 0.473535 |
Target: 5'- cGCCGuuGUagaGAUagaacucgCGCC-CCGCCGCa -3' miRNA: 3'- -CGGCucCGg--CUAga------GCGGuGGCGGCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 26558 | 0.66 | 0.473535 |
Target: 5'- aCCGAGGCUGua---GCCGCCGa-GCa -3' miRNA: 3'- cGGCUCCGGCuagagCGGUGGCggCG- -5' |
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23220 | 5' | -61.6 | NC_005259.1 | + | 2944 | 0.66 | 0.473535 |
Target: 5'- uCCaAGGCgcgCGAgaccCUgGCCACCGCgCGCg -3' miRNA: 3'- cGGcUCCG---GCUa---GAgCGGUGGCG-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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