Results 1 - 20 of 75 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23351 | 5' | -56 | NC_005259.1 | + | 64594 | 0.66 | 0.78469 |
Target: 5'- aGCACACCaacgaggugacugaCGGCGAgCGCGACgGGc -3' miRNA: 3'- gCGUGUGGag------------GCUGUUgGUGCUGgCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 53355 | 0.66 | 0.78373 |
Target: 5'- uCGCGCccGCCU-CGACGGCUAa-ACCGAg -3' miRNA: 3'- -GCGUG--UGGAgGCUGUUGGUgcUGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 21234 | 0.66 | 0.78373 |
Target: 5'- aGUACGCCU-CGGCAGCgCGCGGguuCUGGa -3' miRNA: 3'- gCGUGUGGAgGCUGUUG-GUGCU---GGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 27524 | 0.66 | 0.78373 |
Target: 5'- cCGCACACg-CCGuCGAggccCCACGGCgGGa -3' miRNA: 3'- -GCGUGUGgaGGCuGUU----GGUGCUGgCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 67717 | 0.66 | 0.78373 |
Target: 5'- gGCAUACCcgucagccucgaUCCGAUAcCgGCGGCCa- -3' miRNA: 3'- gCGUGUGG------------AGGCUGUuGgUGCUGGcu -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 14508 | 0.66 | 0.780842 |
Target: 5'- cCGCAUGCacaCCGGCGAgCACGcggugcguuucgguGCCGAg -3' miRNA: 3'- -GCGUGUGga-GGCUGUUgGUGC--------------UGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 59384 | 0.66 | 0.774053 |
Target: 5'- cCGCACcguGCCUCCGcgcGCGAgCGUGACCu- -3' miRNA: 3'- -GCGUG---UGGAGGC---UGUUgGUGCUGGcu -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 5878 | 0.66 | 0.774053 |
Target: 5'- aGCACAUCaacgaucaugCCGuCGACCACG-UCGAg -3' miRNA: 3'- gCGUGUGGa---------GGCuGUUGGUGCuGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 15028 | 0.66 | 0.774053 |
Target: 5'- uCGaCAguCCgaucuaCCGGCAggguGCCAUGGCCGAu -3' miRNA: 3'- -GC-GUguGGa-----GGCUGU----UGGUGCUGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 60180 | 0.66 | 0.774053 |
Target: 5'- gGCgaACGCCUgcucgaCCGACcgGGCCACGaucucGCCGAu -3' miRNA: 3'- gCG--UGUGGA------GGCUG--UUGGUGC-----UGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 15149 | 0.66 | 0.774053 |
Target: 5'- gGC-CACCUCgGugG-CCGaGGCCGAg -3' miRNA: 3'- gCGuGUGGAGgCugUuGGUgCUGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 24641 | 0.66 | 0.764238 |
Target: 5'- -cCGCACC-CCGACGACgA-GACUGAc -3' miRNA: 3'- gcGUGUGGaGGCUGUUGgUgCUGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 38731 | 0.66 | 0.763249 |
Target: 5'- uGC-CGCCguaCCGGCGguggcccGCCGCaGGCCGGu -3' miRNA: 3'- gCGuGUGGa--GGCUGU-------UGGUG-CUGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 18479 | 0.66 | 0.754294 |
Target: 5'- cCGUGC-CCgacgCCGACgAGCUACG-CCGAc -3' miRNA: 3'- -GCGUGuGGa---GGCUG-UUGGUGCuGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 23790 | 0.66 | 0.754294 |
Target: 5'- aCGCACGaaaC-CCaACAACCACGACaggaGAc -3' miRNA: 3'- -GCGUGUg--GaGGcUGUUGGUGCUGg---CU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 18264 | 0.66 | 0.754294 |
Target: 5'- cCGCGCcugaACCUgaGACGACCucgACGcCCGAc -3' miRNA: 3'- -GCGUG----UGGAggCUGUUGG---UGCuGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 66995 | 0.66 | 0.744234 |
Target: 5'- uGUACGCCUCaCGcACGACaCGCGugUa- -3' miRNA: 3'- gCGUGUGGAG-GC-UGUUG-GUGCugGcu -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 40339 | 0.66 | 0.744234 |
Target: 5'- cCGgACACCUCgcgccuCGAUgaugAACCGCG-CCGAg -3' miRNA: 3'- -GCgUGUGGAG------GCUG----UUGGUGCuGGCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 52903 | 0.66 | 0.734068 |
Target: 5'- aCGgGCACCUgCUcACGACCGcCGACuCGAu -3' miRNA: 3'- -GCgUGUGGA-GGcUGUUGGU-GCUG-GCU- -5' |
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23351 | 5' | -56 | NC_005259.1 | + | 51556 | 0.66 | 0.734068 |
Target: 5'- uGCACgacguugggGCCaacgaUCCGGCAGCCGguGCCGAc -3' miRNA: 3'- gCGUG---------UGG-----AGGCUGUUGGUgcUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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