Results 21 - 40 of 82 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23509 | 3' | -50.3 | NC_005259.1 | + | 17739 | 0.68 | 0.928771 |
Target: 5'- uGGcugCUCGaCGCGAucCGuGAGGCCgCCGCg -3' miRNA: 3'- -CCua-GAGC-GCGUU--GCuUUUUGG-GGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 18171 | 0.68 | 0.916873 |
Target: 5'- cGGAUCUgGCaGU--CGAGGucGACCCCGa -3' miRNA: 3'- -CCUAGAgCG-CGuuGCUUU--UUGGGGCg -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 18846 | 0.66 | 0.957795 |
Target: 5'- aGGGUCUCGCcggugccguGCAACGGuu-GCgCgCGUg -3' miRNA: 3'- -CCUAGAGCG---------CGUUGCUuuuUG-GgGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 19862 | 0.71 | 0.813885 |
Target: 5'- uGGcgCUCGCGCccgcCGGGAucgccGACgCCGCg -3' miRNA: 3'- -CCuaGAGCGCGuu--GCUUU-----UUGgGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 20222 | 0.68 | 0.910505 |
Target: 5'- uGGAUCUUGUGgGGauc-GAACaCCCGCa -3' miRNA: 3'- -CCUAGAGCGCgUUgcuuUUUG-GGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 20686 | 0.66 | 0.97186 |
Target: 5'- uGGUCgcacggcaCGCGCucggugguCGAAGGuuucACCCCGCc -3' miRNA: 3'- cCUAGa-------GCGCGuu------GCUUUU----UGGGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 21436 | 0.69 | 0.874581 |
Target: 5'- aGAUCggCGUGCAcAUGGAGAACgCUGCc -3' miRNA: 3'- cCUAGa-GCGCGU-UGCUUUUUGgGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 22942 | 0.71 | 0.804363 |
Target: 5'- cGGGUCaUCGCGcCGAUGAAAcucgucAACCUcaCGCa -3' miRNA: 3'- -CCUAG-AGCGC-GUUGCUUU------UUGGG--GCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 23525 | 0.66 | 0.957795 |
Target: 5'- aGAUCUCGa-CggUGAAAAucaGCCgCCGCc -3' miRNA: 3'- cCUAGAGCgcGuuGCUUUU---UGG-GGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 24071 | 0.71 | 0.813885 |
Target: 5'- -aAUCUCGUGCAccGCGAGAucaCCgGCa -3' miRNA: 3'- ccUAGAGCGCGU--UGCUUUuugGGgCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 24688 | 0.66 | 0.97186 |
Target: 5'- ---cCUCGUGCAcgugccgaGCGAuc-ACCCCGa -3' miRNA: 3'- ccuaGAGCGCGU--------UGCUuuuUGGGGCg -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 25493 | 0.66 | 0.968714 |
Target: 5'- aGGcgCUCGCGCGugaGGgcaaaAAGGCCCuCGa -3' miRNA: 3'- -CCuaGAGCGCGUug-CU-----UUUUGGG-GCg -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 27768 | 0.66 | 0.97186 |
Target: 5'- ---cCUCG-GCGACGAccuuGAGcACCUCGCg -3' miRNA: 3'- ccuaGAGCgCGUUGCU----UUU-UGGGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 28902 | 0.73 | 0.711789 |
Target: 5'- aGAUUgUCG-GCGACGAGGAACgCCGCc -3' miRNA: 3'- cCUAG-AGCgCGUUGCUUUUUGgGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 29953 | 0.66 | 0.970001 |
Target: 5'- cGGucgcggCUgGUGgGGCGGGcggugaccguccaacGGACCCCGCa -3' miRNA: 3'- -CCua----GAgCGCgUUGCUU---------------UUUGGGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 30504 | 0.68 | 0.910505 |
Target: 5'- aGAcCUUGCGCGAUGuguuGAGcucuuuagcGCCCUGCa -3' miRNA: 3'- cCUaGAGCGCGUUGCu---UUU---------UGGGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 31298 | 0.68 | 0.928771 |
Target: 5'- cGGUCU-GCGCAACGAGcuuGCCgagaCCGUu -3' miRNA: 3'- cCUAGAgCGCGUUGCUUuu-UGG----GGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 31845 | 0.67 | 0.954067 |
Target: 5'- --uUCUCGCGCGugaccgcgucauugGCGAucuugucggucguGACCUCGCc -3' miRNA: 3'- ccuAGAGCGCGU--------------UGCUuu-----------UUGGGGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 33385 | 0.67 | 0.953639 |
Target: 5'- cGGUCcgccgaGCGCGACGAGccguguCCaCCGCg -3' miRNA: 3'- cCUAGag----CGCGUUGCUUuuu---GG-GGCG- -5' |
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23509 | 3' | -50.3 | NC_005259.1 | + | 33732 | 0.69 | 0.889752 |
Target: 5'- uGAUgUCGCGUGgagcggugugcACGAGcacGAACaCCCGCa -3' miRNA: 3'- cCUAgAGCGCGU-----------UGCUU---UUUG-GGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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