Results 1 - 20 of 157 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23825 | 3' | -64.1 | NC_005261.1 | + | 37909 | 0.66 | 0.572999 |
Target: 5'- gCCCUACGCGCGCCaCCg--CUgCgCGAg -3' miRNA: 3'- -GGGGUGCGCGCGG-GGaagGGaGgGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 100072 | 0.66 | 0.572999 |
Target: 5'- gCCCGgggGCGCGCCgCgcCCCUCgCCGc -3' miRNA: 3'- gGGGUg--CGCGCGGgGaaGGGAG-GGCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 6848 | 0.66 | 0.572999 |
Target: 5'- aCCCACcCcCGCCCCaUUCaCgCUCCCGu -3' miRNA: 3'- gGGGUGcGcGCGGGG-AAG-G-GAGGGCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 105080 | 0.66 | 0.572999 |
Target: 5'- gUCCGCcgacucCGCGCCCCggaCCUUCCgCGGg -3' miRNA: 3'- gGGGUGc-----GCGCGGGGaa-GGGAGG-GCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 55578 | 0.66 | 0.572999 |
Target: 5'- cCCCCGCaguccgcggcgGCGuCGCCCaugcgCuCCUCCUGGu -3' miRNA: 3'- -GGGGUG-----------CGC-GCGGGgaa--G-GGAGGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 96732 | 0.66 | 0.572999 |
Target: 5'- aCCCGgGCGCGgCgCgagUCCCggCCUGGg -3' miRNA: 3'- gGGGUgCGCGCgGgGa--AGGGa-GGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 77517 | 0.66 | 0.572999 |
Target: 5'- uCgCCGC-CGCGCCCCcg-CC-CCCGAu -3' miRNA: 3'- -GgGGUGcGCGCGGGGaagGGaGGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 39432 | 0.66 | 0.572045 |
Target: 5'- cCCCgGCGCGUccgcaggGCCCCgagCCUCCgCGc -3' miRNA: 3'- -GGGgUGCGCG-------CGGGGaagGGAGG-GCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 69365 | 0.66 | 0.570141 |
Target: 5'- gCCgCGCGCGCGCUggaCCUcgagaugaccgccgUCcugagccuggCCUCCCGAc -3' miRNA: 3'- -GGgGUGCGCGCGG---GGA--------------AG----------GGAGGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 88568 | 0.66 | 0.570141 |
Target: 5'- gCCgGCGaCGCGUCCCgcuucgccgccgaggCCCggCCCGGc -3' miRNA: 3'- gGGgUGC-GCGCGGGGaa-------------GGGa-GGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 117205 | 0.66 | 0.563486 |
Target: 5'- cUCCUACcgGCGCGCCCgCUgCCUgggCgCCGAg -3' miRNA: 3'- -GGGGUG--CGCGCGGG-GAaGGGa--G-GGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 32662 | 0.66 | 0.563486 |
Target: 5'- cCCCCGCGC-CGCuaacgccgccgCCCgccgCCCUCCg-- -3' miRNA: 3'- -GGGGUGCGcGCG-----------GGGaa--GGGAGGgcu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 78136 | 0.66 | 0.563486 |
Target: 5'- aCCCC-CGC-CGCCCCggcgCCCg-CCGc -3' miRNA: 3'- -GGGGuGCGcGCGGGGaa--GGGagGGCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 15125 | 0.66 | 0.563486 |
Target: 5'- aUCCgGCGuCGCGaCUCCUUCgCC-CCCGc -3' miRNA: 3'- -GGGgUGC-GCGC-GGGGAAG-GGaGGGCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 22245 | 0.66 | 0.563486 |
Target: 5'- gCCCCGCcccucCGCGCCCgCUccgcuucuuuuUCCCUCuuCCGc -3' miRNA: 3'- -GGGGUGc----GCGCGGG-GA-----------AGGGAG--GGCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 119855 | 0.66 | 0.563486 |
Target: 5'- gCCCCugacCGUGCGCuCCCUgaccgCCgCagCCGAg -3' miRNA: 3'- -GGGGu---GCGCGCG-GGGAa----GG-GagGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 104609 | 0.66 | 0.563486 |
Target: 5'- -gCCGCGCGUacgcguccaGCCCCUcgagCaCCaUCCCGGa -3' miRNA: 3'- ggGGUGCGCG---------CGGGGAa---G-GG-AGGGCU- -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 127118 | 0.66 | 0.554015 |
Target: 5'- aCCCgcgauuuuuauGCGCGCGCCCg--CCCgCCCc- -3' miRNA: 3'- gGGG-----------UGCGCGCGGGgaaGGGaGGGcu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 32704 | 0.66 | 0.554015 |
Target: 5'- gCCCACcCGC-CCCCcgCCC-CCCGc -3' miRNA: 3'- gGGGUGcGCGcGGGGaaGGGaGGGCu -5' |
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23825 | 3' | -64.1 | NC_005261.1 | + | 82371 | 0.66 | 0.554015 |
Target: 5'- gCgCGCGCGCGCCgCCagcgccgCCCagCCCGc -3' miRNA: 3'- gGgGUGCGCGCGG-GGaa-----GGGa-GGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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