Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25520 | 3' | -48.5 | NC_005337.1 | + | 98460 | 0.66 | 0.999398 |
Target: 5'- uGGcGCGGGAAGuacacguaccgcaucACGAggucCAcCUUCGACg -3' miRNA: 3'- -CCcUGCCCUUU---------------UGCUa---GUaGAAGCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 104300 | 0.66 | 0.999344 |
Target: 5'- aGGACGGGAucACGcccgacGUCAUCaUCaACu -3' miRNA: 3'- cCCUGCCCUuuUGC------UAGUAGaAGcUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 54571 | 0.66 | 0.999344 |
Target: 5'- cGGGcACuGGGGcaAGACGcgCAUCUaCGAg -3' miRNA: 3'- -CCC-UG-CCCU--UUUGCuaGUAGAaGCUg -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 22545 | 0.66 | 0.999344 |
Target: 5'- uGGACGGGAAGACcuUCAcCgcgGACg -3' miRNA: 3'- cCCUGCCCUUUUGcuAGUaGaagCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 108014 | 0.66 | 0.999255 |
Target: 5'- aGGGCGGGcgcgugauggcgcccAAGcagaagauguACGAcaagcacgugcUCAUCUUCGACu -3' miRNA: 3'- cCCUGCCC---------------UUU----------UGCU-----------AGUAGAAGCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 46978 | 0.66 | 0.99919 |
Target: 5'- uGGACauGGAGACGAgcuUCAUCUUCGu- -3' miRNA: 3'- cCCUGccCUUUUGCU---AGUAGAAGCug -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 20791 | 0.66 | 0.99919 |
Target: 5'- gGGGACGGGcgugccGGAGCuggacaggaaGGUCAggaUCUUgGACu -3' miRNA: 3'- -CCCUGCCC------UUUUG----------CUAGU---AGAAgCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 118648 | 0.66 | 0.99919 |
Target: 5'- cGGGGCGGGGcgcGACGcgCA-CggaGACg -3' miRNA: 3'- -CCCUGCCCUu--UUGCuaGUaGaagCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 84746 | 0.66 | 0.99919 |
Target: 5'- cGGGAgauCGaGGAGAACGugugucccAUCGUCaUCGAg -3' miRNA: 3'- -CCCU---GC-CCUUUUGC--------UAGUAGaAGCUg -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 100684 | 0.66 | 0.99919 |
Target: 5'- cGGugcuCGGGcucGAGACGGUCuGUC-UCGGCg -3' miRNA: 3'- cCCu---GCCC---UUUUGCUAG-UAGaAGCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 76924 | 0.66 | 0.999155 |
Target: 5'- gGGGGCGGGAguuacaaAAACGcgCucUCUUuuuaacgCGGCg -3' miRNA: 3'- -CCCUGCCCU-------UUUGCuaGu-AGAA-------GCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 121533 | 0.66 | 0.999006 |
Target: 5'- cGGACGuGGAGACGGaCAUCcucCGGCa -3' miRNA: 3'- cCCUGCcCUUUUGCUaGUAGaa-GCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 55430 | 0.67 | 0.998529 |
Target: 5'- aGGACGGGAAGu---UCAUCaaaGACa -3' miRNA: 3'- cCCUGCCCUUUugcuAGUAGaagCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 67917 | 0.67 | 0.998529 |
Target: 5'- cGGACGGGAGugccGCGGUgGUCgcgcUCcACg -3' miRNA: 3'- cCCUGCCCUUu---UGCUAgUAGa---AGcUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 13900 | 0.67 | 0.998529 |
Target: 5'- -uGACGGGGAccAGCG-UCAUCgUCGGg -3' miRNA: 3'- ccCUGCCCUU--UUGCuAGUAGaAGCUg -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 91610 | 0.67 | 0.997871 |
Target: 5'- cGGGCGcGGAAGcCGAUC-UCgggCGGCc -3' miRNA: 3'- cCCUGC-CCUUUuGCUAGuAGaa-GCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 58431 | 0.68 | 0.996434 |
Target: 5'- cGGAgCGGcGAGACGAUCAgCggCGACc -3' miRNA: 3'- cCCU-GCCcUUUUGCUAGUaGaaGCUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 6491 | 0.68 | 0.995807 |
Target: 5'- cGGACGaGGAGGuCGAUCGgcggCUUCcGCu -3' miRNA: 3'- cCCUGC-CCUUUuGCUAGUa---GAAGcUG- -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 10901 | 0.68 | 0.995807 |
Target: 5'- gGGGACGGGGuacagGGGCGGgggCAggUCcUCGAa -3' miRNA: 3'- -CCCUGCCCU-----UUUGCUa--GU--AGaAGCUg -5' |
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25520 | 3' | -48.5 | NC_005337.1 | + | 47789 | 0.68 | 0.995532 |
Target: 5'- aGGGCGGGGugcugcgcacguGCGAccccUCGcgCUUCGACg -3' miRNA: 3'- cCCUGCCCUuu----------UGCU----AGUa-GAAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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