Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26269 | 5' | -60 | NC_005345.2 | + | 20147 | 0.66 | 0.455032 |
Target: 5'- gUUCGGgagcCACCGCG-CGagGCGGaaGCGGUu -3' miRNA: 3'- -AAGCUa---GUGGCGCuGCa-CGCC--CGCCA- -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 28514 | 0.66 | 0.455032 |
Target: 5'- cUCGAUCAagaCGCaGACGUucGgGGGCGa- -3' miRNA: 3'- aAGCUAGUg--GCG-CUGCA--CgCCCGCca -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 10090 | 0.66 | 0.455032 |
Target: 5'- cUCGAUCGCCGCcuGCGUaGCGcccaccGGcCGGUu -3' miRNA: 3'- aAGCUAGUGGCGc-UGCA-CGC------CC-GCCA- -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 33022 | 0.66 | 0.454062 |
Target: 5'- -aCGAUCgggcucgGCCGCGGgGUGCGGucCGGc -3' miRNA: 3'- aaGCUAG-------UGGCGCUgCACGCCc-GCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 38261 | 0.66 | 0.445391 |
Target: 5'- aUCGAUgACgUGCGG-GUGCGGGCacGGg -3' miRNA: 3'- aAGCUAgUG-GCGCUgCACGCCCG--CCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 31147 | 0.66 | 0.445391 |
Target: 5'- cUCGAUCGCacucgggugCGCGGggucgaaGUGCGGGUGcGUa -3' miRNA: 3'- aAGCUAGUG---------GCGCUg------CACGCCCGC-CA- -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 32790 | 0.66 | 0.445391 |
Target: 5'- -gCGAUCACCGCGGCGc-CGGccGCGa- -3' miRNA: 3'- aaGCUAGUGGCGCUGCacGCC--CGCca -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 864 | 0.66 | 0.445391 |
Target: 5'- -aCGAUCAgCGCGcacugcGCGcgGuCGGGCGGc -3' miRNA: 3'- aaGCUAGUgGCGC------UGCa-C-GCCCGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 43497 | 0.66 | 0.443477 |
Target: 5'- cUCGAUCGCCgccaaggucGCGGCGaacccggcgcucGCGGaGCGGa -3' miRNA: 3'- aAGCUAGUGG---------CGCUGCa-----------CGCC-CGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 22774 | 0.66 | 0.439662 |
Target: 5'- cUCGGUCACCucgggagggauccgcGCGAuccuCGgcgGgGGGCGGg -3' miRNA: 3'- aAGCUAGUGG---------------CGCU----GCa--CgCCCGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 10781 | 0.66 | 0.435867 |
Target: 5'- gUCGAgacaGCCGCGcuGCG-GCaGGCGGa -3' miRNA: 3'- aAGCUag--UGGCGC--UGCaCGcCCGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 34520 | 0.66 | 0.435867 |
Target: 5'- gUCGAggcggGCCGCGGCcucGCGGGCGu- -3' miRNA: 3'- aAGCUag---UGGCGCUGca-CGCCCGCca -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 40495 | 0.66 | 0.435867 |
Target: 5'- --aGAUCGCCGCGACGauCGGcGCcgaGGUg -3' miRNA: 3'- aagCUAGUGGCGCUGCacGCC-CG---CCA- -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 4390 | 0.66 | 0.43209 |
Target: 5'- gUCGGUCACCGUGAUucuccugucagucaGUGCgagcugugccacgGGGCGa- -3' miRNA: 3'- aAGCUAGUGGCGCUG--------------CACG-------------CCCGCca -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 28392 | 0.66 | 0.42646 |
Target: 5'- -aCGcgcCGCCGCGGCGgcagcggcagGCaGGGCGGa -3' miRNA: 3'- aaGCua-GUGGCGCUGCa---------CG-CCCGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 8726 | 0.66 | 0.417176 |
Target: 5'- -cCGAUCGCCGCGuCGgugGCGaGCGcGa -3' miRNA: 3'- aaGCUAGUGGCGCuGCa--CGCcCGC-Ca -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 3936 | 0.66 | 0.417176 |
Target: 5'- gUCGAggugcUCGCCGCGGcCGgcagccucuCGGGCGGc -3' miRNA: 3'- aAGCU-----AGUGGCGCU-GCac-------GCCCGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 31178 | 0.66 | 0.408016 |
Target: 5'- aUCGAg-GCCGCGGCccgGCaGGCGGc -3' miRNA: 3'- aAGCUagUGGCGCUGca-CGcCCGCCa -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 11378 | 0.66 | 0.408016 |
Target: 5'- -cCGGUCGCgGCcGCcUGCGGGCGa- -3' miRNA: 3'- aaGCUAGUGgCGcUGcACGCCCGCca -5' |
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26269 | 5' | -60 | NC_005345.2 | + | 34748 | 0.66 | 0.408016 |
Target: 5'- -gUGAUCcUCGCGGCG-GCG-GCGGUg -3' miRNA: 3'- aaGCUAGuGGCGCUGCaCGCcCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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