Results 1 - 20 of 110 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26668 | 5' | -56.5 | NC_005808.1 | + | 28061 | 0.66 | 0.600687 |
Target: 5'- gACGGUcugcUCGaacaGCGGGCaGguGGCCGCg -3' miRNA: 3'- -UGUCA----AGCcg--CGCCUGcCguUUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 23466 | 0.66 | 0.600687 |
Target: 5'- uACAcgcCGGCGUGGAacaGGUgcGCCGUg -3' miRNA: 3'- -UGUcaaGCCGCGCCUg--CCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 8570 | 0.66 | 0.600687 |
Target: 5'- gGCAGcgacaGGCGCGacacGACGGuCAcggcGACCGUg -3' miRNA: 3'- -UGUCaag--CCGCGC----CUGCC-GU----UUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 27902 | 0.66 | 0.600687 |
Target: 5'- cCAGcaCGuCGCGGAUGGCAcccagcACCGUg -3' miRNA: 3'- uGUCaaGCcGCGCCUGCCGUu-----UGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 22386 | 0.66 | 0.594073 |
Target: 5'- aACAGc-CGGcCGCGGGCgcugccaagaccgagGGCGAcgacaaggcgaaGCCGCa -3' miRNA: 3'- -UGUCaaGCC-GCGCCUG---------------CCGUU------------UGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 5734 | 0.66 | 0.589671 |
Target: 5'- cACGGUUCGGCuaaGUuuguCGGCGAGuuGCu -3' miRNA: 3'- -UGUCAAGCCG---CGccu-GCCGUUUggCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 16140 | 0.66 | 0.589671 |
Target: 5'- gUAGgacugCGGCcgcacgcugaugGUGGugGGCGuuACCGCg -3' miRNA: 3'- uGUCaa---GCCG------------CGCCugCCGUu-UGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 16443 | 0.66 | 0.589671 |
Target: 5'- -----cCGcauCGCaGACGGCGAGCCGCu -3' miRNA: 3'- ugucaaGCc--GCGcCUGCCGUUUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 19363 | 0.66 | 0.589671 |
Target: 5'- cCAGcgaGGCGCGGuCGGUggccgAGGCCGa -3' miRNA: 3'- uGUCaagCCGCGCCuGCCG-----UUUGGCg -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 21445 | 0.66 | 0.588571 |
Target: 5'- uGCAuuuccugCGGCGgGGGCGGCAggauguuggccucGACCauGCg -3' miRNA: 3'- -UGUcaa----GCCGCgCCUGCCGU-------------UUGG--CG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33219 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33267 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33363 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33411 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33315 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33459 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 33507 | 0.66 | 0.586373 |
Target: 5'- cGCGGcaCGGCcaccgcuggcuaucGCGGcACGGCc-ACCGCu -3' miRNA: 3'- -UGUCaaGCCG--------------CGCC-UGCCGuuUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 34883 | 0.66 | 0.578691 |
Target: 5'- aGCGGUgcaggCGGUGUGGGaucacuucgagGGCuaccGGCCGCa -3' miRNA: 3'- -UGUCAa----GCCGCGCCUg----------CCGu---UUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 18529 | 0.66 | 0.578691 |
Target: 5'- cGCGG-UCGccgcuacuguGCGCGGcAUGG-AAACCGCa -3' miRNA: 3'- -UGUCaAGC----------CGCGCC-UGCCgUUUGGCG- -5' |
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26668 | 5' | -56.5 | NC_005808.1 | + | 18064 | 0.66 | 0.578691 |
Target: 5'- -----cCGGCGCGGgguGCGuGCuGGGCCGCc -3' miRNA: 3'- ugucaaGCCGCGCC---UGC-CG-UUUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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