Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26688 | 3' | -57.9 | NC_005808.1 | + | 32934 | 0.66 | 0.503521 |
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Target: 5'- -cGGCUguucGGCCaGGGCGACCACgGCUGc -3' miRNA: 3'- acUUGGu---CUGGgUCCGCUGGUG-CGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 36678 | 0.67 | 0.413797 |
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Target: 5'- aUGAcaACCAGGacuccaauCCC-GGCGgcacacucACCACGCUGg -3' miRNA: 3'- -ACU--UGGUCU--------GGGuCCGC--------UGGUGCGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 37239 | 0.74 | 0.160896 |
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Target: 5'- cUGGGCgCGGugC-AGGCGGCCACGCa- -3' miRNA: 3'- -ACUUG-GUCugGgUCCGCUGGUGCGac -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 37500 | 0.66 | 0.46249 |
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Target: 5'- cGcACCGGACgCUGGGcCGGCCGC-CUGu -3' miRNA: 3'- aCuUGGUCUG-GGUCC-GCUGGUGcGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 38194 | 0.68 | 0.368392 |
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Target: 5'- cGAcuGCCgcacgcucaAGGCCCGGcGCGACgGCGcCUGg -3' miRNA: 3'- aCU--UGG---------UCUGGGUC-CGCUGgUGC-GAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 39410 | 0.68 | 0.394308 |
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Target: 5'- gUGAGCaagcuguacgcagCGGACgCCGGGCuuGCCAUGCUGc -3' miRNA: 3'- -ACUUG-------------GUCUG-GGUCCGc-UGGUGCGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 39744 | 0.71 | 0.24588 |
|
Target: 5'- cGAACCAGGCCCcucgcuggugcuGGCGccGCUGCGCg- -3' miRNA: 3'- aCUUGGUCUGGGu-----------CCGC--UGGUGCGac -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 40837 | 0.68 | 0.377195 |
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Target: 5'- cGAcgcGCCAGGCgCAGGCcGGCCAUGa-- -3' miRNA: 3'- aCU---UGGUCUGgGUCCG-CUGGUGCgac -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 41608 | 0.74 | 0.148094 |
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Target: 5'- gUGAACCAcGGCaccggCCAGGCGGCCAagugGCUGc -3' miRNA: 3'- -ACUUGGU-CUG-----GGUCCGCUGGUg---CGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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