Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27928 | 3' | -56.6 | NC_005886.2 | + | 28784 | 0.66 | 0.637432 |
Target: 5'- --cCACGAugGC-ACGaaCGGUGCGAc -3' miRNA: 3'- gauGUGCUugCGuUGCcgGCCGCGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 27065 | 0.66 | 0.626469 |
Target: 5'- --cCGCGuaGAUGUAgaaACGGCCcGCGCGAu -3' miRNA: 3'- gauGUGC--UUGCGU---UGCCGGcCGCGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 32842 | 0.66 | 0.626469 |
Target: 5'- -cAgACGAACGCAugauCcGCuCGGUGCGGa -3' miRNA: 3'- gaUgUGCUUGCGUu---GcCG-GCCGCGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 19493 | 0.66 | 0.626469 |
Target: 5'- -gGCACGuGGgGCuugcCGGCCGGCuCGAu -3' miRNA: 3'- gaUGUGC-UUgCGuu--GCCGGCCGcGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 11941 | 0.66 | 0.626469 |
Target: 5'- -gGguCGGGCGUggcaAACGGCCGGUG-GAu -3' miRNA: 3'- gaUguGCUUGCG----UUGCCGGCCGCgCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 15147 | 0.66 | 0.625372 |
Target: 5'- aUACAUGAauucgGCGCAccaucgcGCGGCauccccGCGCGAu -3' miRNA: 3'- gAUGUGCU-----UGCGU-------UGCCGgc----CGCGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 7190 | 0.66 | 0.604566 |
Target: 5'- --gUACGAcaACaCGACGGCCGGCaGCu- -3' miRNA: 3'- gauGUGCU--UGcGUUGCCGGCCG-CGcu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 24915 | 0.66 | 0.604566 |
Target: 5'- -aACGCcccGCGCGAcCGGCUcGCGCGGg -3' miRNA: 3'- gaUGUGcu-UGCGUU-GCCGGcCGCGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 43761 | 0.66 | 0.593646 |
Target: 5'- -gACGCuGGCGCAGCGuUgGGCGCGc -3' miRNA: 3'- gaUGUGcUUGCGUUGCcGgCCGCGCu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 11574 | 0.66 | 0.593646 |
Target: 5'- gCUAUGuCGAACuccCGGgGGCCGGUGCGc -3' miRNA: 3'- -GAUGU-GCUUGc--GUUgCCGGCCGCGCu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 46904 | 0.66 | 0.593646 |
Target: 5'- -aGCGCGAuuuucuGCGCGACGagugcGCCGGCaaGCa- -3' miRNA: 3'- gaUGUGCU------UGCGUUGC-----CGGCCG--CGcu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 36330 | 0.66 | 0.587108 |
Target: 5'- -gGCACGGGCgGCAcgggcggugguacggGCGGCauCGGCgGCGGc -3' miRNA: 3'- gaUGUGCUUG-CGU---------------UGCCG--GCCG-CGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 2733 | 0.66 | 0.582758 |
Target: 5'- -aACGguuCGAAucuuCGC-GCGGCCGGCGUGu -3' miRNA: 3'- gaUGU---GCUU----GCGuUGCCGGCCGCGCu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 38819 | 0.66 | 0.582758 |
Target: 5'- gUGCcCGcAGCGCAGCGGUCGccgaGCGGa -3' miRNA: 3'- gAUGuGC-UUGCGUUGCCGGCcg--CGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 22896 | 0.67 | 0.57191 |
Target: 5'- -aACuCGGGCGaUAGCGuGCCGGUGCu- -3' miRNA: 3'- gaUGuGCUUGC-GUUGC-CGGCCGCGcu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 25819 | 0.67 | 0.57191 |
Target: 5'- -aACGCagccauAGCGCAAUGGCUGGCGa-- -3' miRNA: 3'- gaUGUGc-----UUGCGUUGCCGGCCGCgcu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 33146 | 0.67 | 0.561111 |
Target: 5'- -aACGCuGcAugGCAugGGCCGGU-CGAu -3' miRNA: 3'- gaUGUG-C-UugCGUugCCGGCCGcGCU- -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 42918 | 0.67 | 0.550369 |
Target: 5'- -cGCACGuucgauGCGCAACaauuccguGUCGGCGCGu -3' miRNA: 3'- gaUGUGCu-----UGCGUUGc-------CGGCCGCGCu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 16691 | 0.67 | 0.533319 |
Target: 5'- -cGCugGAaaucaucgcagaccuGCGCAACGGCCuuaccGCGCu- -3' miRNA: 3'- gaUGugCU---------------UGCGUUGCCGGc----CGCGcu -5' |
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27928 | 3' | -56.6 | NC_005886.2 | + | 24759 | 0.67 | 0.529085 |
Target: 5'- -cGCGCGGACGCGgggcuucuaGCGGCCGucaaGCa- -3' miRNA: 3'- gaUGUGCUUGCGU---------UGCCGGCcg--CGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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