Results 1 - 20 of 55 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28158 | 5' | -55.2 | NC_005887.1 | + | 27422 | 0.66 | 0.658932 |
Target: 5'- uGCCGAcGCucGUCUGCgcgcCGCUgcgcgUGGCGc -3' miRNA: 3'- gCGGCU-CG--UAGACGau--GUGAa----GCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 34292 | 0.66 | 0.658932 |
Target: 5'- uCGCCGuGCggCUGaCUGCGgg-CGGCa -3' miRNA: 3'- -GCGGCuCGuaGAC-GAUGUgaaGCCGc -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 30367 | 0.66 | 0.658932 |
Target: 5'- uGCCGAGC-UCgGUguacCGC-UCGGCGu -3' miRNA: 3'- gCGGCUCGuAGaCGau--GUGaAGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 28270 | 0.66 | 0.647711 |
Target: 5'- cCGCCGAGacgC-GCgGCGCUgaugCGGCGc -3' miRNA: 3'- -GCGGCUCguaGaCGaUGUGAa---GCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 1548 | 0.66 | 0.647711 |
Target: 5'- cCGCgCGAGCG-CcGC-ACGCcgUCGGCGu -3' miRNA: 3'- -GCG-GCUCGUaGaCGaUGUGa-AGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 26109 | 0.66 | 0.636475 |
Target: 5'- gCGUCGAGCAggaugucCUGCacggcGCGCUUCGuuucGCGg -3' miRNA: 3'- -GCGGCUCGUa------GACGa----UGUGAAGC----CGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 18997 | 0.66 | 0.636475 |
Target: 5'- cCGCCGAcgGCcgCUGCgaga-UUCGGUGa -3' miRNA: 3'- -GCGGCU--CGuaGACGaugugAAGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 40609 | 0.66 | 0.614001 |
Target: 5'- uCGaCGAGCGUgUGUggccGCACaUCGGCa -3' miRNA: 3'- -GCgGCUCGUAgACGa---UGUGaAGCCGc -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 31904 | 0.66 | 0.614001 |
Target: 5'- aGCCGgucaGGCAUgCUGCUGCAagaUCcgGGCGu -3' miRNA: 3'- gCGGC----UCGUA-GACGAUGUga-AG--CCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 2432 | 0.66 | 0.614001 |
Target: 5'- gCGCCGAcgaauGaCAcCUcGCUGCGCUaCGGCGc -3' miRNA: 3'- -GCGGCU-----C-GUaGA-CGAUGUGAaGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 41999 | 0.66 | 0.614001 |
Target: 5'- aCGCCGGGCAgcucagCUGCgagccgcucaGCACgugCGGgGu -3' miRNA: 3'- -GCGGCUCGUa-----GACGa---------UGUGaa-GCCgC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 21575 | 0.67 | 0.591593 |
Target: 5'- aGCCG-GCGUC-GUUGCGCcgauugcCGGCGg -3' miRNA: 3'- gCGGCuCGUAGaCGAUGUGaa-----GCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 28472 | 0.67 | 0.590476 |
Target: 5'- uGCCGGGCcgCUucaGCUcgacgaaguagacGCGCgcCGGCGg -3' miRNA: 3'- gCGGCUCGuaGA---CGA-------------UGUGaaGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 21403 | 0.67 | 0.584895 |
Target: 5'- cCGCCG-GCAUucgguaacaccacacCUGCcGCugUuUCGGCGa -3' miRNA: 3'- -GCGGCuCGUA---------------GACGaUGugA-AGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 41413 | 0.67 | 0.584895 |
Target: 5'- uGCCGAGCAggucgaauUCUuccagcgccucguggGCUauGCGCUgcUCGGCa -3' miRNA: 3'- gCGGCUCGU--------AGA---------------CGA--UGUGA--AGCCGc -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 5722 | 0.67 | 0.580438 |
Target: 5'- uCGCCGGuGCcgCaUGCUuCGCUUCGaGCu -3' miRNA: 3'- -GCGGCU-CGuaG-ACGAuGUGAAGC-CGc -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 32833 | 0.67 | 0.569327 |
Target: 5'- gCGCaCGAGCcggCUGCaugGCGCgcgcCGGCGc -3' miRNA: 3'- -GCG-GCUCGua-GACGa--UGUGaa--GCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 15077 | 0.67 | 0.547274 |
Target: 5'- aGCgCGAGCAUCaGCUcgggcgGCACgccugCGGCc -3' miRNA: 3'- gCG-GCUCGUAGaCGA------UGUGaa---GCCGc -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 40451 | 0.68 | 0.536348 |
Target: 5'- uGCCGGGCAUCacgauuuccucGC-GCGCgaCGGCGg -3' miRNA: 3'- gCGGCUCGUAGa----------CGaUGUGaaGCCGC- -5' |
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28158 | 5' | -55.2 | NC_005887.1 | + | 28335 | 0.68 | 0.536348 |
Target: 5'- uGUCG-GCGUCUGCgccgGCGCggcaUUGGCu -3' miRNA: 3'- gCGGCuCGUAGACGa---UGUGa---AGCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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