Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2884 | 5' | -49.8 | NC_001493.1 | + | 28475 | 0.66 | 0.996581 |
Target: 5'- gUGAGUUcaUCGcgcaggaccACCCGAUCCCGu---- -3' miRNA: 3'- gAUUCAG--AGC---------UGGGCUAGGGCuuuua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 35199 | 0.66 | 0.996581 |
Target: 5'- -gGAGcgCuUCGACCCGAgggaCCUGGAGGUc -3' miRNA: 3'- gaUUCa-G-AGCUGGGCUa---GGGCUUUUA- -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 110509 | 0.66 | 0.996581 |
Target: 5'- -aAGGUCga-GCCCGcgCCCGGAGu- -3' miRNA: 3'- gaUUCAGagcUGGGCuaGGGCUUUua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 95853 | 0.66 | 0.995972 |
Target: 5'- -aGAGUCaCGAucgcaacaucCCCGcUCCCGAGGAa -3' miRNA: 3'- gaUUCAGaGCU----------GGGCuAGGGCUUUUa -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 21924 | 0.66 | 0.995277 |
Target: 5'- -cAGGUUUccCGACCCGGguacgUCCCGAu--- -3' miRNA: 3'- gaUUCAGA--GCUGGGCU-----AGGGCUuuua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 32726 | 0.66 | 0.994816 |
Target: 5'- --cGGUC-CGGCCCGAgaccccuucuucgucUCCCGGGc-- -3' miRNA: 3'- gauUCAGaGCUGGGCU---------------AGGGCUUuua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 67026 | 0.66 | 0.994488 |
Target: 5'- ---uGUUUCGugCCGAUcagcCCCGGGAu- -3' miRNA: 3'- gauuCAGAGCugGGCUA----GGGCUUUua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 133267 | 0.66 | 0.994488 |
Target: 5'- ---cGUCUCG-CCCGGUCCgugCGAGu-- -3' miRNA: 3'- gauuCAGAGCuGGGCUAGG---GCUUuua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 17713 | 0.66 | 0.994488 |
Target: 5'- ---cGUCUCG-CCCGGUCCgugCGAGu-- -3' miRNA: 3'- gauuCAGAGCuGGGCUAGG---GCUUuua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 19957 | 0.66 | 0.993595 |
Target: 5'- ----cUCUgG-UCCGGUCCCGAAGAUg -3' miRNA: 3'- gauucAGAgCuGGGCUAGGGCUUUUA- -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 59883 | 0.66 | 0.993595 |
Target: 5'- -cGGG-CUaccCGACCCcuauGAUCCCGAAAAUu -3' miRNA: 3'- gaUUCaGA---GCUGGG----CUAGGGCUUUUA- -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 4300 | 0.67 | 0.99259 |
Target: 5'- gUGGGggUUCGuuguucccacCCCGGUCCCGAGGAg -3' miRNA: 3'- gAUUCa-GAGCu---------GGGCUAGGGCUUUUa -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 119854 | 0.67 | 0.99259 |
Target: 5'- gUGGGggUUCGuuguucccacCCCGGUCCCGAGGAg -3' miRNA: 3'- gAUUCa-GAGCu---------GGGCUAGGGCUUUUa -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 13578 | 0.67 | 0.991464 |
Target: 5'- -cGAGaC-CGACCCG-UCCCGAGGc- -3' miRNA: 3'- gaUUCaGaGCUGGGCuAGGGCUUUua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 26374 | 0.67 | 0.991464 |
Target: 5'- -cGGGUUUCGGCCCcagCCCGGu--- -3' miRNA: 3'- gaUUCAGAGCUGGGcuaGGGCUuuua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 129132 | 0.67 | 0.991464 |
Target: 5'- -cGAGaC-CGACCCG-UCCCGAGGc- -3' miRNA: 3'- gaUUCaGaGCUGGGCuAGGGCUUUua -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 110791 | 0.67 | 0.990206 |
Target: 5'- -cGAGUUUCGAagcggUCGAUCCCGGcgGUa -3' miRNA: 3'- gaUUCAGAGCUg----GGCUAGGGCUuuUA- -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 9124 | 0.68 | 0.983668 |
Target: 5'- -gGGGUCauccccgCGACCgCGcUCCCGggGAUg -3' miRNA: 3'- gaUUCAGa------GCUGG-GCuAGGGCuuUUA- -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 124679 | 0.68 | 0.983668 |
Target: 5'- -gGGGUCauccccgCGACCgCGcUCCCGggGAUg -3' miRNA: 3'- gaUUCAGa------GCUGG-GCuAGGGCuuUUA- -5' |
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2884 | 5' | -49.8 | NC_001493.1 | + | 88507 | 0.68 | 0.981609 |
Target: 5'- gUGGGUUgacacaCGACCCGAaccUCUCGGAAAg -3' miRNA: 3'- gAUUCAGa-----GCUGGGCU---AGGGCUUUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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