Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3586 | 3' | -58.7 | NC_001650.1 | + | 6854 | 0.66 | 0.831076 |
Target: 5'- gCCCUgGUuGCUGGGCAGacuguGGGGGAc- -3' miRNA: 3'- aGGGGgCAcCGACCCGUU-----CUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 90194 | 0.66 | 0.831076 |
Target: 5'- gCCCCC-UGGCggcggugGGgaaGCGGGAGAGGg- -3' miRNA: 3'- aGGGGGcACCGa------CC---CGUUCUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 131545 | 0.66 | 0.831076 |
Target: 5'- gCCgCCCGggGGCUGGacGCGGGgcGGAGAa- -3' miRNA: 3'- aGG-GGGCa-CCGACC--CGUUC--UCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 173561 | 0.66 | 0.831076 |
Target: 5'- gCCCUgGUuGCUGGGCAGacuguGGGGGAc- -3' miRNA: 3'- aGGGGgCAcCGACCCGUU-----CUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 2174 | 0.66 | 0.822776 |
Target: 5'- cUCCCCauUGGCuaaaUGGGuCAGuGGGAGGUAc -3' miRNA: 3'- -AGGGGgcACCG----ACCC-GUU-CUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 146153 | 0.66 | 0.822776 |
Target: 5'- cUCgCCCGggccgGGCUgGGGCAcAGGGAGc-- -3' miRNA: 3'- -AGgGGGCa----CCGA-CCCGU-UCUCUCuau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 136286 | 0.66 | 0.822776 |
Target: 5'- gCCCCCGccuucaUGGCggccgcgGGGCu-GGGGGAg- -3' miRNA: 3'- aGGGGGC------ACCGa------CCCGuuCUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 168881 | 0.66 | 0.822776 |
Target: 5'- cUCCCCauUGGCuaaaUGGGuCAGuGGGAGGUAc -3' miRNA: 3'- -AGGGGgcACCG----ACCC-GUU-CUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 59285 | 0.66 | 0.814308 |
Target: 5'- cCCCCCGagauauggaGGCUGGuguacgacggcuGCAAG-GAGAUGg -3' miRNA: 3'- aGGGGGCa--------CCGACC------------CGUUCuCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 8001 | 0.66 | 0.814308 |
Target: 5'- aCCCCC-UGGuCUGccuGGCAaccAGAGAGAc- -3' miRNA: 3'- aGGGGGcACC-GAC---CCGU---UCUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 174708 | 0.66 | 0.814308 |
Target: 5'- aCCCCC-UGGuCUGccuGGCAaccAGAGAGAc- -3' miRNA: 3'- aGGGGGcACC-GAC---CCGU---UCUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 175251 | 0.66 | 0.805681 |
Target: 5'- aCCCCCGgGGUgguggGGGCuauGGGGGUGu -3' miRNA: 3'- aGGGGGCaCCGa----CCCGuucUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 8544 | 0.66 | 0.805681 |
Target: 5'- aCCCCCGgGGUgguggGGGCuauGGGGGUGu -3' miRNA: 3'- aGGGGGCaCCGa----CCCGuucUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 132446 | 0.66 | 0.805681 |
Target: 5'- gCCgCC-UGGCUGGGgGAGAG-GAa- -3' miRNA: 3'- aGGgGGcACCGACCCgUUCUCuCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 1831 | 0.66 | 0.796902 |
Target: 5'- aUCCCCUcucGGCcaaugGGGguAGGGGGGUGg -3' miRNA: 3'- -AGGGGGca-CCGa----CCCguUCUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 168538 | 0.66 | 0.796902 |
Target: 5'- aUCCCCUcucGGCcaaugGGGguAGGGGGGUGg -3' miRNA: 3'- -AGGGGGca-CCGa----CCCguUCUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 102532 | 0.66 | 0.78708 |
Target: 5'- gUCCCCCGggaucuGCUGGGCGGGucccauaaaguacAGAGc-- -3' miRNA: 3'- -AGGGGGCac----CGACCCGUUC-------------UCUCuau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 162856 | 0.67 | 0.778921 |
Target: 5'- uUUCUCCGccauuGCUGGGCuuGGAGGGGUGc -3' miRNA: 3'- -AGGGGGCac---CGACCCGu-UCUCUCUAU- -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 75087 | 0.67 | 0.778921 |
Target: 5'- -aCCCCG-GGagcGGaGCGAGAGAGAa- -3' miRNA: 3'- agGGGGCaCCga-CC-CGUUCUCUCUau -5' |
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3586 | 3' | -58.7 | NC_001650.1 | + | 131740 | 0.67 | 0.778008 |
Target: 5'- gCCCCCGggcGGCcacguugaccgcgUGGGCgAAGAGGGu-- -3' miRNA: 3'- aGGGGGCa--CCG-------------ACCCG-UUCUCUCuau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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