Results 1 - 20 of 71 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3586 | 5' | -64.6 | NC_001650.1 | + | 38655 | 0.66 | 0.604376 |
Target: 5'- gGCGCCccCUCCCGguugcggagcGGCGGCGcCacaGCGg -3' miRNA: 3'- gCGCGGc-GAGGGU----------CCGUCGCaGg--CGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 123058 | 0.66 | 0.594885 |
Target: 5'- uGgGCUGCUgC-AGGCucucuaugaaGGCGUCCGUGg -3' miRNA: 3'- gCgCGGCGAgGgUCCG----------UCGCAGGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 172420 | 0.66 | 0.594885 |
Target: 5'- uGgGCCGCUCCUcccacaagauGGCAGCGcCCu-- -3' miRNA: 3'- gCgCGGCGAGGGu---------CCGUCGCaGGcgc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 5713 | 0.66 | 0.594885 |
Target: 5'- uGgGCCGCUCCUcccacaagauGGCAGCGcCCu-- -3' miRNA: 3'- gCgCGGCGAGGGu---------CCGUCGCaGGcgc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 68852 | 0.66 | 0.585417 |
Target: 5'- aCGUGCCGCgcCCCGGGCccauggCCGCc -3' miRNA: 3'- -GCGCGGCGa-GGGUCCGucgca-GGCGc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 121422 | 0.66 | 0.585417 |
Target: 5'- gGCaCCGCUCCaggaAGGCcuccacCGUCCGCc -3' miRNA: 3'- gCGcGGCGAGGg---UCCGuc----GCAGGCGc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 80258 | 0.66 | 0.575979 |
Target: 5'- aCGgGCUGCUgCUggAGGCGuGCGUCCcCGa -3' miRNA: 3'- -GCgCGGCGAgGG--UCCGU-CGCAGGcGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 146468 | 0.66 | 0.575979 |
Target: 5'- -cCGCgGC-CCUAGGCGGCccgggcgCCGCGa -3' miRNA: 3'- gcGCGgCGaGGGUCCGUCGca-----GGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 46371 | 0.66 | 0.575979 |
Target: 5'- gGCGCCGCaucggCCgCgGGGCAccagauGCG-CCGCGg -3' miRNA: 3'- gCGCGGCGa----GG-G-UCCGU------CGCaGGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 109585 | 0.66 | 0.575979 |
Target: 5'- -aUGCCGgUUCCAGG-GGCccGUCCGCGu -3' miRNA: 3'- gcGCGGCgAGGGUCCgUCG--CAGGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 143105 | 0.66 | 0.575979 |
Target: 5'- gCGCGCCgGCUgCaCGGGCcggGGCGgCgGCGg -3' miRNA: 3'- -GCGCGG-CGAgG-GUCCG---UCGCaGgCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 38440 | 0.66 | 0.575037 |
Target: 5'- gCGcCGCCGCUCCgCaaccgggAGGgGGCGcCUGUGa -3' miRNA: 3'- -GC-GCGGCGAGG-G-------UCCgUCGCaGGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 165155 | 0.66 | 0.570332 |
Target: 5'- aCGCGCCGgaCCggggaGccggggugagagacuGGCGGCGgCCGCGg -3' miRNA: 3'- -GCGCGGCgaGGg----U---------------CCGUCGCaGGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 60516 | 0.66 | 0.566575 |
Target: 5'- gGCGCCGgggUCCUGGGCAGaggCgGCGg -3' miRNA: 3'- gCGCGGCg--AGGGUCCGUCgcaGgCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 42852 | 0.66 | 0.566575 |
Target: 5'- uGCGUgGC-CCCGGGCAcGUGUgUCGCc -3' miRNA: 3'- gCGCGgCGaGGGUCCGU-CGCA-GGCGc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 30885 | 0.66 | 0.566575 |
Target: 5'- -cCGCuCGC-CCCGcGGCGcgcGCGUCCGCc -3' miRNA: 3'- gcGCG-GCGaGGGU-CCGU---CGCAGGCGc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 81628 | 0.66 | 0.557211 |
Target: 5'- gCGCGCCGCg--CGGGcCAGCucccccgucacGUUCGCGg -3' miRNA: 3'- -GCGCGGCGaggGUCC-GUCG-----------CAGGCGC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 136639 | 0.66 | 0.557211 |
Target: 5'- gGCGCCcugagGCUCCgCGGGCagagugccaGGCGagCCGCc -3' miRNA: 3'- gCGCGG-----CGAGG-GUCCG---------UCGCa-GGCGc -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 136827 | 0.66 | 0.557211 |
Target: 5'- gGCGCCccgacGCcCCCGGGCGGUGggCUGgGu -3' miRNA: 3'- gCGCGG-----CGaGGGUCCGUCGCa-GGCgC- -5' |
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3586 | 5' | -64.6 | NC_001650.1 | + | 51065 | 0.67 | 0.547892 |
Target: 5'- uGCGCUGCgCCagcagaaaGGGCAGCucgCCGCc -3' miRNA: 3'- gCGCGGCGaGGg-------UCCGUCGca-GGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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