Results 21 - 40 of 69 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5519 | 5' | -59.2 | NC_001798.1 | + | 31246 | 0.69 | 0.607348 |
Target: 5'- gCGCCC-CGUugCGGGCGggGGUGGGAUc -3' miRNA: 3'- -GCGGGcGCG--GCCCGCgaUUACUCUAc -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 31783 | 0.67 | 0.716735 |
Target: 5'- aCGCgCGCgggucgggaggGCCGGGCGCggaggGAGGa- -3' miRNA: 3'- -GCGgGCG-----------CGGCCCGCGauua-CUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 32261 | 0.77 | 0.234386 |
Target: 5'- gCGUCCGCGUCcGGCGCUGggGAGAg- -3' miRNA: 3'- -GCGGGCGCGGcCCGCGAUuaCUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 32789 | 0.66 | 0.788954 |
Target: 5'- gCGCCCGCGCCGGccggcgacgcccccGCggGCcAcgGGGAg- -3' miRNA: 3'- -GCGGGCGCGGCC--------------CG--CGaUuaCUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 33137 | 0.66 | 0.791631 |
Target: 5'- -cCCCGCGCagGGGgGCcgggGAUGGGggGg -3' miRNA: 3'- gcGGGCGCGg-CCCgCGa---UUACUCuaC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 34873 | 0.77 | 0.240011 |
Target: 5'- cCGCCCgGCGCCGGGCgGCUAcccGGGAc- -3' miRNA: 3'- -GCGGG-CGCGGCCCG-CGAUua-CUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 39844 | 0.69 | 0.617353 |
Target: 5'- cCGCCgCGCuGCCGGGCgaggcgucgagGCUucgGGGGUGc -3' miRNA: 3'- -GCGG-GCG-CGGCCCG-----------CGAuuaCUCUAC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 40497 | 0.66 | 0.791631 |
Target: 5'- uGCCUGUGCUGGGC-CggggGAGGg- -3' miRNA: 3'- gCGGGCGCGGCCCGcGauuaCUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 52079 | 0.67 | 0.745578 |
Target: 5'- cCGCCCGCuacGCgGGGCGC--AUGAcGUa -3' miRNA: 3'- -GCGGGCG---CGgCCCGCGauUACUcUAc -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 52764 | 0.72 | 0.453718 |
Target: 5'- uCGcCCCGCGuccucggcgccCCGGGCGCccGUGAGcgGg -3' miRNA: 3'- -GC-GGGCGC-----------GGCCCGCGauUACUCuaC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 55975 | 0.73 | 0.401583 |
Target: 5'- aGCCCGCGCCGGGgaggcCGCgggGGcGAGGg- -3' miRNA: 3'- gCGGGCGCGGCCC-----GCGa--UUaCUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 56407 | 0.7 | 0.546943 |
Target: 5'- gGuCCCGCGgCGGGgGCUGGUGGcugccguGGUGc -3' miRNA: 3'- gC-GGGCGCgGCCCgCGAUUACU-------CUAC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 59459 | 0.71 | 0.499838 |
Target: 5'- -cCCCGCGugcCCGGGCGC-GGUGAGGc- -3' miRNA: 3'- gcGGGCGC---GGCCCGCGaUUACUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 59700 | 0.67 | 0.716735 |
Target: 5'- aCGCCCGUcuUCGGGCGCUu----GGUGg -3' miRNA: 3'- -GCGGGCGc-GGCCCGCGAuuacuCUAC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 66869 | 0.67 | 0.736047 |
Target: 5'- uCGCCgggGCGCCGGG-GCU--UGGGAa- -3' miRNA: 3'- -GCGGg--CGCGGCCCgCGAuuACUCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 71103 | 0.66 | 0.800461 |
Target: 5'- gCGUgCGCGCCcccgGGGaCGCUGAUGGc--- -3' miRNA: 3'- -GCGgGCGCGG----CCC-GCGAUUACUcuac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 72154 | 0.69 | 0.597361 |
Target: 5'- gGCCgGCGUCGGGCccacGAUGcAGAUGg -3' miRNA: 3'- gCGGgCGCGGCCCGcga-UUAC-UCUAC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 75988 | 0.66 | 0.764345 |
Target: 5'- uGCCCGCcgccCCGGGCGCcgGcgGcAGGg- -3' miRNA: 3'- gCGGGCGc---GGCCCGCGa-UuaC-UCUac -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 79180 | 0.74 | 0.323653 |
Target: 5'- cCGCCCuguGCGCCcGGCuGCggGAUGAGGUGg -3' miRNA: 3'- -GCGGG---CGCGGcCCG-CGa-UUACUCUAC- -5' |
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5519 | 5' | -59.2 | NC_001798.1 | + | 79444 | 0.66 | 0.791631 |
Target: 5'- aCGCCCGCGCCcGGGCcuCUucuucccccGAGGg- -3' miRNA: 3'- -GCGGGCGCGG-CCCGc-GAuua------CUCUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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