Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5540 | 5' | -53.5 | NC_001806.1 | + | 136042 | 0.67 | 0.930841 |
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Target: 5'- -cGGCACCgGcCACCCcgcGCUCGCUGUc -3' miRNA: 3'- aaUUGUGGgU-GUGGGu--UGGGUGACAc -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 143908 | 0.7 | 0.814848 |
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Target: 5'- ---cCACCCAC-CCCAcgccCCCACUGa- -3' miRNA: 3'- aauuGUGGGUGuGGGUu---GGGUGACac -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 143955 | 0.68 | 0.872859 |
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Target: 5'- ---cCGCCCACGCCCccGCCC-CUGc- -3' miRNA: 3'- aauuGUGGGUGUGGGu-UGGGuGACac -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 144310 | 0.67 | 0.936024 |
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Target: 5'- -cGACACCCcCcCCCcGCCCG-UGUGg -3' miRNA: 3'- aaUUGUGGGuGuGGGuUGGGUgACAC- -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 145447 | 0.66 | 0.945629 |
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Target: 5'- gUAACccCCCACGCCaauGACCCACg--- -3' miRNA: 3'- aAUUGu-GGGUGUGGg--UUGGGUGacac -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 146114 | 1.07 | 0.005678 |
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Target: 5'- cUUAACACCCACACCCAACCCACUGUGg -3' miRNA: 3'- -AAUUGUGGGUGUGGGUUGGGUGACAC- -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 149639 | 0.67 | 0.924846 |
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Target: 5'- aUGGCGCCCcaacaugGCGCCCGGCUC-CcGUGu -3' miRNA: 3'- aAUUGUGGG-------UGUGGGUUGGGuGaCAC- -5' |
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| 5540 | 5' | -53.5 | NC_001806.1 | + | 149762 | 0.71 | 0.728054 |
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Target: 5'- aUGGCGCCCcagacaugGCGCCCGGCCCcucaccucgcGCUGg- -3' miRNA: 3'- aAUUGUGGG--------UGUGGGUUGGG----------UGACac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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