Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5545 | 5' | -52.4 | NC_001806.1 | + | 151547 | 0.71 | 0.872225 |
Target: 5'- gCCcGGGCgg-CCGggGGCggCGGGGGc -3' miRNA: 3'- -GGuUCUGuaaGGCuuCUGa-GCCCCCu -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 150627 | 0.71 | 0.872225 |
Target: 5'- cCCAauGGGCcgggccCCGAAGACUCGGcGGAc -3' miRNA: 3'- -GGU--UCUGuaa---GGCUUCUGAGCCcCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 149011 | 0.69 | 0.934536 |
Target: 5'- aCCcAGACA-UCCGGggcGGGCUaaGGGGAa -3' miRNA: 3'- -GGuUCUGUaAGGCU---UCUGAgcCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 147763 | 0.66 | 0.989161 |
Target: 5'- cCCGAcGCgg-CCGcGGACgCGGGGGGc -3' miRNA: 3'- -GGUUcUGuaaGGCuUCUGaGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 147278 | 0.72 | 0.832605 |
Target: 5'- gCgGAGGCcg-CCGAGGACgucaGGGGGGu -3' miRNA: 3'- -GgUUCUGuaaGGCUUCUGag--CCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 146774 | 0.67 | 0.967113 |
Target: 5'- uCCGAcGCGggggCCGucgGGuACUCGGGGGGc -3' miRNA: 3'- -GGUUcUGUaa--GGCu--UC-UGAGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 144020 | 0.66 | 0.986115 |
Target: 5'- cCCAacGGAaagcggCGggGugUUGGGGGAg -3' miRNA: 3'- -GGU--UCUguaag-GCuuCugAGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 141615 | 1.12 | 0.005113 |
Target: 5'- gCCAAGACAUUCCGAAGACUCGGGGGAg -3' miRNA: 3'- -GGUUCUGUAAGGCUUCUGAGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 138550 | 0.66 | 0.985434 |
Target: 5'- uUCGAGGCAguguugcgugaCGAcagcuuuguaaAGACUCaGGGGGAg -3' miRNA: 3'- -GGUUCUGUaag--------GCU-----------UCUGAG-CCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 129695 | 0.74 | 0.710533 |
Target: 5'- uCC-GGACGccucUCCGGAGGCcCGGGGGGc -3' miRNA: 3'- -GGuUCUGUa---AGGCUUCUGaGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 126874 | 0.67 | 0.978107 |
Target: 5'- -gAGGACGgcgCCGggGcuCUCGGGGc- -3' miRNA: 3'- ggUUCUGUaa-GGCuuCu-GAGCCCCcu -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 125548 | 0.67 | 0.978107 |
Target: 5'- cCCcAGACAUUCCGcuauCUCcuGGGGGc -3' miRNA: 3'- -GGuUCUGUAAGGCuucuGAG--CCCCCu -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 124086 | 0.74 | 0.720597 |
Target: 5'- cCCGGGGC---CCGAAGcUUCGGGGGGu -3' miRNA: 3'- -GGUUCUGuaaGGCUUCuGAGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 118582 | 0.71 | 0.879513 |
Target: 5'- cCCAcGcCcUUcCCGcGGACUCGGGGGAc -3' miRNA: 3'- -GGUuCuGuAA-GGCuUCUGAGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 117958 | 0.66 | 0.985434 |
Target: 5'- cCCGAcGACAgcgacgccggucCCGGAGGCUCGGGu-- -3' miRNA: 3'- -GGUU-CUGUaa----------GGCUUCUGAGCCCccu -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 117423 | 0.68 | 0.950214 |
Target: 5'- uCCGAGGCGccguuccguuUUCCgGAauacguacggacgucAGugUUGGGGGAg -3' miRNA: 3'- -GGUUCUGU----------AAGG-CU---------------UCugAGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 115233 | 0.72 | 0.824092 |
Target: 5'- cCgGGGACGcgccgCCGggGGCguuuacgCGGGGGAc -3' miRNA: 3'- -GgUUCUGUaa---GGCuuCUGa------GCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 113381 | 0.67 | 0.967113 |
Target: 5'- uCCGGcGGCGUUCCG-GGACgccCGGGGc- -3' miRNA: 3'- -GGUU-CUGUAAGGCuUCUGa--GCCCCcu -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 113301 | 0.69 | 0.929391 |
Target: 5'- -gGGGACGUUCCcccgGccGGCcCGGGGGAg -3' miRNA: 3'- ggUUCUGUAAGG----CuuCUGaGCCCCCU- -5' |
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5545 | 5' | -52.4 | NC_001806.1 | + | 106830 | 0.7 | 0.906368 |
Target: 5'- cCCAguuccugucgcGGGCAUaCgCGGAGuuuuuCUCGGGGGAc -3' miRNA: 3'- -GGU-----------UCUGUAaG-GCUUCu----GAGCCCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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