Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5545 | 5' | -52.4 | NC_001806.1 | + | 147278 | 0.72 | 0.832605 |
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Target: 5'- gCgGAGGCcg-CCGAGGACgucaGGGGGGu -3' miRNA: 3'- -GgUUCUGuaaGGCUUCUGag--CCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 115233 | 0.72 | 0.824092 |
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Target: 5'- cCgGGGACGcgccgCCGggGGCguuuacgCGGGGGAc -3' miRNA: 3'- -GgUUCUGUaa---GGCuuCUGa------GCCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 124086 | 0.74 | 0.720597 |
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Target: 5'- cCCGGGGC---CCGAAGcUUCGGGGGGu -3' miRNA: 3'- -GGUUCUGuaaGGCUUCuGAGCCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 129695 | 0.74 | 0.710533 |
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Target: 5'- uCC-GGACGccucUCCGGAGGCcCGGGGGGc -3' miRNA: 3'- -GGuUCUGUa---AGGCUUCUGaGCCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 77126 | 0.75 | 0.69021 |
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Target: 5'- gCCGAG-CGggCCGggGccguggaggaGCUCGGGGGGc -3' miRNA: 3'- -GGUUCuGUaaGGCuuC----------UGAGCCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 38384 | 0.77 | 0.546259 |
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Target: 5'- uCCAGGuCGUcgcggcggcucUCCGccGGCUCGGGGGGg -3' miRNA: 3'- -GGUUCuGUA-----------AGGCuuCUGAGCCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 76120 | 0.8 | 0.413088 |
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Target: 5'- aCGAGGCcgUCCuacaccugucgGAAGACUUGGGGGGc -3' miRNA: 3'- gGUUCUGuaAGG-----------CUUCUGAGCCCCCU- -5' |
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| 5545 | 5' | -52.4 | NC_001806.1 | + | 141615 | 1.12 | 0.005113 |
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Target: 5'- gCCAAGACAUUCCGAAGACUCGGGGGAg -3' miRNA: 3'- -GGUUCUGUAAGGCUUCUGAGCCCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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