Results 1 - 20 of 93 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5648 | 3' | -57.5 | NC_001806.1 | + | 150092 | 0.68 | 0.690438 |
Target: 5'- gGGAGGCAgGCCCAccgcggggcGGCCC-CGUCCcCg -3' miRNA: 3'- gUCUUCGU-UGGGU---------UCGGGaGCGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 149935 | 0.69 | 0.65985 |
Target: 5'- aGGggGCGGCCCGAGUCU--GCCUg- -3' miRNA: 3'- gUCuuCGUUGGGUUCGGGagCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 149802 | 0.67 | 0.759669 |
Target: 5'- uGGggGCGGCCCucAGGCCggcggguaCUCGCUC-Cg -3' miRNA: 3'- gUCuuCGUUGGG--UUCGG--------GAGCGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 149421 | 0.66 | 0.805897 |
Target: 5'- uCAGGgggucccacGGCGAcCCCGGGCCCUgCGUCUg- -3' miRNA: 3'- -GUCU---------UCGUU-GGGUUCGGGA-GCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 147507 | 0.68 | 0.700548 |
Target: 5'- aGGggGCGGCgCCGcgggagGGCCCgUGCCCa- -3' miRNA: 3'- gUCuuCGUUG-GGU------UCGGGaGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 147297 | 0.66 | 0.796934 |
Target: 5'- uCAGggGgGuCCCGGGCCCacccuggccgCGCCCc- -3' miRNA: 3'- -GUCuuCgUuGGGUUCGGGa---------GCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 143946 | 0.66 | 0.805897 |
Target: 5'- --cGAGCAcccccGCCCAcGCCCcCGCCCc- -3' miRNA: 3'- gucUUCGU-----UGGGUuCGGGaGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 143427 | 0.66 | 0.82334 |
Target: 5'- --cGAGCGGCCC-GGCCCgCGCUCcCa -3' miRNA: 3'- gucUUCGUUGGGuUCGGGaGCGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 140242 | 0.66 | 0.840083 |
Target: 5'- gCGGGAGauucaaCUGAGCCC-CGCCCUg -3' miRNA: 3'- -GUCUUCguug--GGUUCGGGaGCGGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 138686 | 0.75 | 0.318702 |
Target: 5'- uGGAuGCGACCCAgaGGaCCCUCGCCUa- -3' miRNA: 3'- gUCUuCGUUGGGU--UC-GGGAGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 135980 | 0.68 | 0.740318 |
Target: 5'- -cGAGGCGGCCgAcccGGCCUuggucucgUCGUCCUCc -3' miRNA: 3'- guCUUCGUUGGgU---UCGGG--------AGCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 134995 | 0.67 | 0.759669 |
Target: 5'- ----cGguGCCCGAuGCCCcCGCCCUg -3' miRNA: 3'- gucuuCguUGGGUU-CGGGaGCGGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 132687 | 0.66 | 0.805008 |
Target: 5'- uGGggGCcuGGCCCGcggugggGGCCagggUCGUCCUCc -3' miRNA: 3'- gUCuuCG--UUGGGU-------UCGGg---AGCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 125570 | 0.68 | 0.740318 |
Target: 5'- uGGggGCA-CCCAcGCCCgCGaCCCg- -3' miRNA: 3'- gUCuuCGUuGGGUuCGGGaGC-GGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 125414 | 0.68 | 0.729508 |
Target: 5'- cCAGAcgacaucAGCugugGACCCGAGCUCccauUCGCCCg- -3' miRNA: 3'- -GUCU-------UCG----UUGGGUUCGGG----AGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 123231 | 0.68 | 0.730495 |
Target: 5'- gAGAuGCcGCCCAcgcGGCCCUgCGCCgaCg -3' miRNA: 3'- gUCUuCGuUGGGU---UCGGGA-GCGGgaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 117686 | 0.74 | 0.356772 |
Target: 5'- gAGAAGCucggGGCCCGAGCCCgcgUCGgccCCCUCc -3' miRNA: 3'- gUCUUCG----UUGGGUUCGGG---AGC---GGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 116836 | 0.69 | 0.663945 |
Target: 5'- gGGAGGCcGCCCAgcucauucaccccgaAGCCC-CGgCCCUg -3' miRNA: 3'- gUCUUCGuUGGGU---------------UCGGGaGC-GGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 115308 | 0.68 | 0.690438 |
Target: 5'- cCAG-AGCGACCCGucccgGGCCUUCGCggCCa- -3' miRNA: 3'- -GUCuUCGUUGGGU-----UCGGGAGCG--GGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 112019 | 0.68 | 0.730495 |
Target: 5'- uCAGccuGGCcACCgAGGCCaUCGCCCUg -3' miRNA: 3'- -GUCu--UCGuUGGgUUCGGgAGCGGGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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