Results 21 - 40 of 93 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5648 | 3' | -57.5 | NC_001806.1 | + | 20569 | 0.69 | 0.65985 |
Target: 5'- -cGggGCGcggACCCAcccGCCCUCGCgCCcCg -3' miRNA: 3'- guCuuCGU---UGGGUu--CGGGAGCG-GGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 116836 | 0.69 | 0.663945 |
Target: 5'- gGGAGGCcGCCCAgcucauucaccccgaAGCCC-CGgCCCUg -3' miRNA: 3'- gUCUUCGuUGGGU---------------UCGGGaGC-GGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 46673 | 0.69 | 0.680278 |
Target: 5'- --cGAGCGACCuCGAGCCC-CaGCCCg- -3' miRNA: 3'- gucUUCGUUGG-GUUCGGGaG-CGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 88998 | 0.68 | 0.689424 |
Target: 5'- cCAGAAGCcGCCCGA-CCCgacgcggaggacgUCGCgCUCg -3' miRNA: 3'- -GUCUUCGuUGGGUUcGGG-------------AGCGgGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 76353 | 0.68 | 0.690438 |
Target: 5'- gCAGggGUcACCCu-GCCCU-GCCCg- -3' miRNA: 3'- -GUCuuCGuUGGGuuCGGGAgCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 115308 | 0.68 | 0.690438 |
Target: 5'- cCAG-AGCGACCCGucccgGGCCUUCGCggCCa- -3' miRNA: 3'- -GUCuUCGUUGGGU-----UCGGGAGCG--GGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 69898 | 0.68 | 0.690438 |
Target: 5'- -cGcGGCGACCCAGauccgcuuCCC-CGCCCUCg -3' miRNA: 3'- guCuUCGUUGGGUUc-------GGGaGCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 2312 | 0.68 | 0.690438 |
Target: 5'- -cGAGGCcccgccgccGgCCAGGUCCUCGCCCg- -3' miRNA: 3'- guCUUCGu--------UgGGUUCGGGAGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 150092 | 0.68 | 0.690438 |
Target: 5'- gGGAGGCAgGCCCAccgcggggcGGCCC-CGUCCcCg -3' miRNA: 3'- gUCUUCGU-UGGGU---------UCGGGaGCGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 96492 | 0.68 | 0.69954 |
Target: 5'- gCGGccGCGACCCAggcggcgaacgggGGCCCU-GCCaCUCc -3' miRNA: 3'- -GUCuuCGUUGGGU-------------UCGGGAgCGG-GAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 83575 | 0.68 | 0.700548 |
Target: 5'- aAGAAgGCGACCCuggucGGCuCCUucgcacgcuuUGCCCUCa -3' miRNA: 3'- gUCUU-CGUUGGGu----UCG-GGA----------GCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 147507 | 0.68 | 0.700548 |
Target: 5'- aGGggGCGGCgCCGcgggagGGCCCgUGCCCa- -3' miRNA: 3'- gUCuuCGUUG-GGU------UCGGGaGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 1448 | 0.68 | 0.700548 |
Target: 5'- cCGGcGGCAGggccCCCGGGCCgUCGUCgUCg -3' miRNA: 3'- -GUCuUCGUU----GGGUUCGGgAGCGGgAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 5608 | 0.68 | 0.707591 |
Target: 5'- uGGccGCGGCCCGuuggucgaacccccGGCCC-CGCCCa- -3' miRNA: 3'- gUCuuCGUUGGGU--------------UCGGGaGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 43869 | 0.68 | 0.710601 |
Target: 5'- cCAGAccuacAGCcucCCCAAccGCaCCUCGCCCUg -3' miRNA: 3'- -GUCU-----UCGuu-GGGUU--CG-GGAGCGGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 91174 | 0.68 | 0.720586 |
Target: 5'- aCGGccGCGGCCCGcGCCaaCGCCgUCg -3' miRNA: 3'- -GUCuuCGUUGGGUuCGGgaGCGGgAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 41004 | 0.68 | 0.720586 |
Target: 5'- gGGAuguguccauGGC-GCCCGGGUCCUCcGCCCa- -3' miRNA: 3'- gUCU---------UCGuUGGGUUCGGGAG-CGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 73996 | 0.68 | 0.720586 |
Target: 5'- -cGggGCGGCCCcgcaggGGGCUCcgCGCCCa- -3' miRNA: 3'- guCuuCGUUGGG------UUCGGGa-GCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 125414 | 0.68 | 0.729508 |
Target: 5'- cCAGAcgacaucAGCugugGACCCGAGCUCccauUCGCCCg- -3' miRNA: 3'- -GUCU-------UCG----UUGGGUUCGGG----AGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 123231 | 0.68 | 0.730495 |
Target: 5'- gAGAuGCcGCCCAcgcGGCCCUgCGCCgaCg -3' miRNA: 3'- gUCUuCGuUGGGU---UCGGGA-GCGGgaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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