Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6365 | 3' | -48.9 | NC_001847.1 | + | 2200 | 0.69 | 0.981278 |
Target: 5'- uGCGGGCGCaCCUcaaacaugcggUAGAGGUcGcgGCCGu -3' miRNA: 3'- uUGCCUGCG-GGA-----------AUUUUUA-CuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 4820 | 0.79 | 0.56042 |
Target: 5'- cGCGcGAUGCCCUUGAGcGUGggGCCGc -3' miRNA: 3'- uUGC-CUGCGGGAAUUUuUACuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 6744 | 0.67 | 0.991396 |
Target: 5'- uAGCGGGCGUCgUcuGcgGUGcgGCCGg -3' miRNA: 3'- -UUGCCUGCGGgAauUuuUACuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 11951 | 0.7 | 0.967554 |
Target: 5'- cAUGGACGCCgcUAGGGAUGGgcgGCUGa -3' miRNA: 3'- uUGCCUGCGGgaAUUUUUACUa--CGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 12048 | 0.69 | 0.970745 |
Target: 5'- -cUGGACGCCCUgcgcgcuGcgGAcGCCGa -3' miRNA: 3'- uuGCCUGCGGGAauuu---UuaCUaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 12582 | 0.66 | 0.998002 |
Target: 5'- uACGcGGCGCCCUcgcacucGUGcgGCCa -3' miRNA: 3'- uUGC-CUGCGGGAauuuu--UACuaCGGc -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 14820 | 0.66 | 0.99777 |
Target: 5'- uGCGcGGCGCCCUgcgcgcggUGGAAaaguauauguauuacAUGcgGCCGg -3' miRNA: 3'- uUGC-CUGCGGGA--------AUUUU---------------UACuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 16996 | 0.67 | 0.992547 |
Target: 5'- --aGGACGUCCUUGGcAAcGgcGCCGa -3' miRNA: 3'- uugCCUGCGGGAAUUuUUaCuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 21022 | 0.7 | 0.967554 |
Target: 5'- uGCGGGCGaCCCUgcgcagagAGAGAUGggGCUc -3' miRNA: 3'- uUGCCUGC-GGGAa-------UUUUUACuaCGGc -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 27534 | 0.66 | 0.998002 |
Target: 5'- --gGGACGCCCga-GAAcgGcgGCgCGg -3' miRNA: 3'- uugCCUGCGGGaauUUUuaCuaCG-GC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 30315 | 0.66 | 0.996427 |
Target: 5'- uGCGGACGCCCgcccccccgGcgGCgGg -3' miRNA: 3'- uUGCCUGCGGGaauuuuua-CuaCGgC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 31357 | 0.67 | 0.992867 |
Target: 5'- cGGCGGAaGCCCcgccggcccgGGUGCCGg -3' miRNA: 3'- -UUGCCUgCGGGaauuuuua--CUACGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 31883 | 0.69 | 0.976441 |
Target: 5'- cGGCGGA-GCCCgucgcGcgGAUGCCGc -3' miRNA: 3'- -UUGCCUgCGGGaauuuUuaCUACGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 33398 | 0.68 | 0.987086 |
Target: 5'- gAGCuGGACGCCgUgcuGAGUGAcgugGCCGc -3' miRNA: 3'- -UUG-CCUGCGGgAauuUUUACUa---CGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 34364 | 0.67 | 0.992547 |
Target: 5'- cGGCGGGCGCU------GAUGcgGCCGa -3' miRNA: 3'- -UUGCCUGCGGgaauuuUUACuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 37550 | 0.66 | 0.995987 |
Target: 5'- cGACGGGCgGCCCUUcgGAAGAcGcgGCgGc -3' miRNA: 3'- -UUGCCUG-CGGGAA--UUUUUaCuaCGgC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 40392 | 0.68 | 0.985145 |
Target: 5'- cGACGGccccggccaagacGCGCCCaaagcccGAGUGGUGCCu -3' miRNA: 3'- -UUGCC-------------UGCGGGaauu---UUUACUACGGc -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 49573 | 0.66 | 0.997603 |
Target: 5'- cGACGG-CGCCgUUGAGAcgacgGCCGa -3' miRNA: 3'- -UUGCCuGCGGgAAUUUUuacuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 49711 | 0.7 | 0.964124 |
Target: 5'- gGGCGcGGCGCCCgUGcAAGUGGgcGCCGg -3' miRNA: 3'- -UUGC-CUGCGGGaAUuUUUACUa-CGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 52047 | 0.66 | 0.995987 |
Target: 5'- aGGCGGAgGCCCgcccGGAGGUGuggGCgGg -3' miRNA: 3'- -UUGCCUgCGGGaa--UUUUUACua-CGgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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