Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7183 | 3' | -55.3 | NC_001900.1 | + | 17749 | 0.66 | 0.698514 |
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Target: 5'- cCCUCGGcACUGCgcuuACCACUgcgcugcaGGCGa -3' miRNA: 3'- -GGAGCCaUGACGac--UGGUGGag------CUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 15255 | 0.66 | 0.698514 |
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Target: 5'- --gUGGUcCUGCUGcACCAgUUCGAUGa -3' miRNA: 3'- ggaGCCAuGACGAC-UGGUgGAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 14303 | 0.67 | 0.655076 |
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Target: 5'- aCggCGGUGC-GCUGACCgguCUCGAUGc -3' miRNA: 3'- gGa-GCCAUGaCGACUGGug-GAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 10503 | 0.69 | 0.535689 |
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Target: 5'- gCCUCGGc-CUGCUGGgCACgauggUCGACGc -3' miRNA: 3'- -GGAGCCauGACGACUgGUGg----AGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 9417 | 0.67 | 0.644138 |
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Target: 5'- aCC-CGGUGCUGU---UCGCgCUCGACGc -3' miRNA: 3'- -GGaGCCAUGACGacuGGUG-GAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 6128 | 0.67 | 0.655076 |
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Target: 5'- aCCaacGUGCUGCUGAgCCACUucccguacgUCGGCGa -3' miRNA: 3'- -GGagcCAUGACGACU-GGUGG---------AGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 5281 | 0.67 | 0.665993 |
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Target: 5'- aCCgagCGGUagcACUGCUGcCgGCCgugcgUGACGg -3' miRNA: 3'- -GGa--GCCA---UGACGACuGgUGGa----GCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 3004 | 0.66 | 0.708171 |
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Target: 5'- cUCUCcGcacUGCUGCUGACCgaaggcgACCggCGACGg -3' miRNA: 3'- -GGAGcC---AUGACGACUGG-------UGGa-GCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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