Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7194 | 5' | -55.9 | NC_001900.1 | + | 35746 | 0.72 | 0.325766 |
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Target: 5'- uGGCCGuAGCcaGggGUgAacaCGCCGCCGa -3' miRNA: 3'- -CCGGCuUCGa-CuuUAgUg--GCGGCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 12953 | 0.73 | 0.287814 |
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Target: 5'- uGUCGAGGCUGAGuacgcguUCACUGCaacgaCGCCGa -3' miRNA: 3'- cCGGCUUCGACUUu------AGUGGCG-----GCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 42568 | 0.73 | 0.280654 |
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Target: 5'- gGGCCG-AGCUGggGg-ACCGC-GCCGu -3' miRNA: 3'- -CCGGCuUCGACuuUagUGGCGgCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 44895 | 0.73 | 0.273637 |
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Target: 5'- uGCCGGuagacgacgcgAGUuguuUGggGUCACCGCCgGCCGc -3' miRNA: 3'- cCGGCU-----------UCG----ACuuUAGUGGCGG-CGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 25056 | 0.74 | 0.22841 |
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Target: 5'- cGGCCGc-GCUGGuagCACCuguGCCGCCGu -3' miRNA: 3'- -CCGGCuuCGACUuuaGUGG---CGGCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 28915 | 0.79 | 0.12218 |
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Target: 5'- aGGCCGcgcAGacgGAAGUCGCCGUCGCCa -3' miRNA: 3'- -CCGGCu--UCga-CUUUAGUGGCGGCGGc -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 5428 | 0.85 | 0.042166 |
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Target: 5'- -cCCGAAGCUGAAGUCGCCGUacgCGCCGc -3' miRNA: 3'- ccGGCUUCGACUUUAGUGGCG---GCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 26461 | 1.11 | 0.000563 |
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Target: 5'- uGGCCGAAGCUGAAAUCACCGCCGCCGa -3' miRNA: 3'- -CCGGCUUCGACUUUAGUGGCGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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