Results 1 - 20 of 72 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7198 | 3' | -55.6 | NC_001900.1 | + | 967 | 0.66 | 0.694646 |
Target: 5'- gCUGCUGGCCuacGGCCGGcugcGGUACUgCGCg -3' miRNA: 3'- -GGUGGCCGGu--UUGGCCa---CUAUGG-GUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 1213 | 0.68 | 0.53099 |
Target: 5'- gCACCGGCaacgCAGgcACCGGUGGUguACUCGg -3' miRNA: 3'- gGUGGCCG----GUU--UGGCCACUA--UGGGUg -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 3282 | 0.7 | 0.439155 |
Target: 5'- gUCACCGGCCGcgacuuccGCUGGUcGAUccCCCACc -3' miRNA: 3'- -GGUGGCCGGUu-------UGGCCA-CUAu-GGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 3503 | 0.7 | 0.439155 |
Target: 5'- cCCGCagGGUCuGGCCGGUGAcAUCCAg -3' miRNA: 3'- -GGUGg-CCGGuUUGGCCACUaUGGGUg -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 3928 | 0.72 | 0.324211 |
Target: 5'- -aGCUGGCCGAgacGCCGGU---ACCCGCc -3' miRNA: 3'- ggUGGCCGGUU---UGGCCAcuaUGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 4138 | 0.66 | 0.672852 |
Target: 5'- gCCgACCGGCgAAgcgGCCGGcGGUGacCCCAa -3' miRNA: 3'- -GG-UGGCCGgUU---UGGCCaCUAU--GGGUg -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 4185 | 0.66 | 0.705458 |
Target: 5'- uCUACCGGCaaccCCGGUaGGcGCaCCACu -3' miRNA: 3'- -GGUGGCCGguuuGGCCA-CUaUG-GGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 6006 | 0.75 | 0.22718 |
Target: 5'- -aAUCGGCgCGAGCCGGUGAccUCCGCg -3' miRNA: 3'- ggUGGCCG-GUUUGGCCACUauGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 7142 | 0.69 | 0.489154 |
Target: 5'- gCCAUCGGCCGGAUC-GUGAUgaagGCCaGCg -3' miRNA: 3'- -GGUGGCCGGUUUGGcCACUA----UGGgUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 8639 | 0.66 | 0.661891 |
Target: 5'- gCACCGGCgCcccGCUGGUGuu-CCUGCa -3' miRNA: 3'- gGUGGCCG-Guu-UGGCCACuauGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 8778 | 0.71 | 0.383212 |
Target: 5'- gCCgACCGGCCAca-CGGUcGAggacaugACCCGCg -3' miRNA: 3'- -GG-UGGCCGGUuugGCCA-CUa------UGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 9769 | 0.7 | 0.419985 |
Target: 5'- cCCACgGGCuCGGcgcggucCCGGUGGU-CCCGCu -3' miRNA: 3'- -GGUGgCCG-GUUu------GGCCACUAuGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 12246 | 0.67 | 0.633291 |
Target: 5'- aCCACgGGCCAGAagaucCCGcacugggucggcgacGUGAgUGCCCAg -3' miRNA: 3'- -GGUGgCCGGUUU-----GGC---------------CACU-AUGGGUg -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 13452 | 0.68 | 0.552396 |
Target: 5'- gUCACCGGCCuGAUCGGUGGcgaUGagUACg -3' miRNA: 3'- -GGUGGCCGGuUUGGCCACU---AUggGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 14242 | 0.68 | 0.552396 |
Target: 5'- aCCGa-GGUCuccgagauccuGAGCCGGUG-UGCCCGCg -3' miRNA: 3'- -GGUggCCGG-----------UUUGGCCACuAUGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 17259 | 0.66 | 0.683773 |
Target: 5'- uUCACCgGGCuCGGGCCGGUcaucucgACCgGCa -3' miRNA: 3'- -GGUGG-CCG-GUUUGGCCAcua----UGGgUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 17466 | 0.72 | 0.348642 |
Target: 5'- -aACCGGCCGAACCGccaGAaGCUCACc -3' miRNA: 3'- ggUGGCCGGUUUGGCca-CUaUGGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 18024 | 0.68 | 0.541658 |
Target: 5'- -uGCCGGUCAucguGCCGGUGG--UCCAg -3' miRNA: 3'- ggUGGCCGGUu---UGGCCACUauGGGUg -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 18348 | 0.72 | 0.348642 |
Target: 5'- aCCcCCGGCCuuucgGGGCCGGUGGguucGgCCACu -3' miRNA: 3'- -GGuGGCCGG-----UUUGGCCACUa---UgGGUG- -5' |
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7198 | 3' | -55.6 | NC_001900.1 | + | 18580 | 0.66 | 0.694646 |
Target: 5'- aCCGCUGGUgcccgacgUGAGCCGGccguUGGUGCCgUACg -3' miRNA: 3'- -GGUGGCCG--------GUUUGGCC----ACUAUGG-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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