Results 1 - 20 of 58 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7347 | 3' | -55.3 | NC_001900.1 | + | 44406 | 0.66 | 0.72149 |
Target: 5'- cGAUCGCUGGCGUaCCGu-CCGggGUc -3' miRNA: 3'- aCUAGUGGCUGCAcGGCuuGGCgaCG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 27998 | 0.66 | 0.72149 |
Target: 5'- cGAaCA-CGACGUGCgGAGCCcucggGCUGg -3' miRNA: 3'- aCUaGUgGCUGCACGgCUUGG-----CGACg -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 37400 | 0.66 | 0.720422 |
Target: 5'- aGGUCGCCGAUGUGgaCG-ACCucgucggGCUGg -3' miRNA: 3'- aCUAGUGGCUGCACg-GCuUGG-------CGACg -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 43480 | 0.66 | 0.710767 |
Target: 5'- gGAUCgGCCGGCcaGCuCGGugcGCCGcCUGCu -3' miRNA: 3'- aCUAG-UGGCUGcaCG-GCU---UGGC-GACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 44921 | 0.66 | 0.699966 |
Target: 5'- gGGUCACCGcCG-GCCGcuUCGCcggucgGCa -3' miRNA: 3'- aCUAGUGGCuGCaCGGCuuGGCGa-----CG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 42736 | 0.66 | 0.699966 |
Target: 5'- uUGA-CAgCGGcCGUgGCCGGugCgGCUGCg -3' miRNA: 3'- -ACUaGUgGCU-GCA-CGGCUugG-CGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 46133 | 0.66 | 0.698883 |
Target: 5'- -cAUCGCCGAgcUG-GUCGAugaccagGCCGUUGCg -3' miRNA: 3'- acUAGUGGCU--GCaCGGCU-------UGGCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 42775 | 0.66 | 0.689099 |
Target: 5'- uUGAgagCGCCagcgacGAUGUcGCCGAuguucGCgGCUGCg -3' miRNA: 3'- -ACUa--GUGG------CUGCA-CGGCU-----UGgCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 2226 | 0.66 | 0.689099 |
Target: 5'- aGuUC-CCGACG-GCCGug-CGCUGCc -3' miRNA: 3'- aCuAGuGGCUGCaCGGCuugGCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 9077 | 0.66 | 0.689099 |
Target: 5'- aGA-UACCGGCGagaaGCUGAGCUuccuuggaauGCUGCg -3' miRNA: 3'- aCUaGUGGCUGCa---CGGCUUGG----------CGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 15029 | 0.66 | 0.678177 |
Target: 5'- -cGUCGCCGAa--GCaGGcACCGCUGCa -3' miRNA: 3'- acUAGUGGCUgcaCGgCU-UGGCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 19676 | 0.66 | 0.678177 |
Target: 5'- aGAUCGCuCGACugaugGCCGAGgagauggguCCGgUGCu -3' miRNA: 3'- aCUAGUG-GCUGca---CGGCUU---------GGCgACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 37085 | 0.66 | 0.667211 |
Target: 5'- gGGUCACCGuugaGCGgcaguuccUGCCGAccACCGaaGCc -3' miRNA: 3'- aCUAGUGGC----UGC--------ACGGCU--UGGCgaCG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 18584 | 0.66 | 0.667211 |
Target: 5'- cUGGUgC-CCGACGUgaGCCG-GCCGUUGg -3' miRNA: 3'- -ACUA-GuGGCUGCA--CGGCuUGGCGACg -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 46985 | 0.66 | 0.667211 |
Target: 5'- --uUCugUG-CGUGCagguaGACCGCUGCg -3' miRNA: 3'- acuAGugGCuGCACGgc---UUGGCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 20217 | 0.67 | 0.656212 |
Target: 5'- cGAg-ACCGGCGUaGCCa---CGCUGCa -3' miRNA: 3'- aCUagUGGCUGCA-CGGcuugGCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 25742 | 0.67 | 0.656212 |
Target: 5'- --cUCGCCGACGcggGCCGcGCCcCgGCg -3' miRNA: 3'- acuAGUGGCUGCa--CGGCuUGGcGaCG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 27894 | 0.67 | 0.656212 |
Target: 5'- --uUCAcaguCCGAUc-GCUGGGCCGCUGCc -3' miRNA: 3'- acuAGU----GGCUGcaCGGCUUGGCGACG- -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 25767 | 0.67 | 0.656212 |
Target: 5'- cGAcCGCCGugGUGuuGAcUCGCUc- -3' miRNA: 3'- aCUaGUGGCugCACggCUuGGCGAcg -5' |
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7347 | 3' | -55.3 | NC_001900.1 | + | 1353 | 0.67 | 0.656212 |
Target: 5'- gUGGUCAacgucCCGACcu-CCGAgAUCGCUGCa -3' miRNA: 3'- -ACUAGU-----GGCUGcacGGCU-UGGCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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