Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8073 | 3' | -61.8 | NC_001978.2 | + | 33872 | 0.66 | 0.373031 |
Target: 5'- aCCGuGCGcguguaCGCCGACgACGACCCu--- -3' miRNA: 3'- -GGC-CGCag----GCGGCUG-UGCUGGGccau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 22617 | 0.66 | 0.356412 |
Target: 5'- aCCGGCacUCaG-CGACGCG-CCCGGUAg -3' miRNA: 3'- -GGCCGc-AGgCgGCUGUGCuGGGCCAU- -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 740 | 0.66 | 0.356412 |
Target: 5'- uUGGCGUaggCGCCGAaggugaagccgACGAgCCGGUAa -3' miRNA: 3'- gGCCGCAg--GCGGCUg----------UGCUgGGCCAU- -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 38588 | 0.66 | 0.356412 |
Target: 5'- gUCGGCGUUCGggaCGGCggaGCG-CCCGGa- -3' miRNA: 3'- -GGCCGCAGGCg--GCUG---UGCuGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 35581 | 0.66 | 0.3483 |
Target: 5'- aCGGCGcCCcugagcGUCGGCGCGAccgaagacCCCGGg- -3' miRNA: 3'- gGCCGCaGG------CGGCUGUGCU--------GGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 35993 | 0.66 | 0.332474 |
Target: 5'- cCCGGaGaCUGCCG-C-CGACCCGGg- -3' miRNA: 3'- -GGCCgCaGGCGGCuGuGCUGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 34136 | 0.66 | 0.332474 |
Target: 5'- cCCGgguguGCGUCCGCCGGaGCGugCCcacGUGg -3' miRNA: 3'- -GGC-----CGCAGGCGGCUgUGCugGGc--CAU- -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 17530 | 0.66 | 0.324761 |
Target: 5'- gCGGC-UCCGUCucCGcCGACCCGGa- -3' miRNA: 3'- gGCCGcAGGCGGcuGU-GCUGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 2430 | 0.66 | 0.3483 |
Target: 5'- gCGGaCGUCCGCCauaGGCACGcGCCaauGGg- -3' miRNA: 3'- gGCC-GCAGGCGG---CUGUGC-UGGg--CCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 10696 | 0.66 | 0.353965 |
Target: 5'- aCCGGCGcUgagcaacgacgacgCUGCCGACaggauGCGACCgGGa- -3' miRNA: 3'- -GGCCGC-A--------------GGCGGCUG-----UGCUGGgCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 32713 | 0.66 | 0.373031 |
Target: 5'- -gGGCGUCagcgucaGUCGGC-CGGuCCCGGUu -3' miRNA: 3'- ggCCGCAGg------CGGCUGuGCU-GGGCCAu -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 32471 | 0.66 | 0.364656 |
Target: 5'- aCGGUG-CCGacucgaCCGACugACGACCUGGg- -3' miRNA: 3'- gGCCGCaGGC------GGCUG--UGCUGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 38367 | 0.67 | 0.279247 |
Target: 5'- -gGGCGUagggUCGCCGACAUGACacaagggguugugaCCGGg- -3' miRNA: 3'- ggCCGCA----GGCGGCUGUGCUG--------------GGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 19534 | 0.67 | 0.281294 |
Target: 5'- aCCGGCG-CCGaCCacauCACG-CCCGGg- -3' miRNA: 3'- -GGCCGCaGGC-GGcu--GUGCuGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 32358 | 0.67 | 0.302427 |
Target: 5'- --aGCGUCCGCCGA-ACGGCUCGa-- -3' miRNA: 3'- ggcCGCAGGCGGCUgUGCUGGGCcau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 11048 | 0.67 | 0.309738 |
Target: 5'- gCGGaCGUCU-CCGGCGUGAUCCGGUu -3' miRNA: 3'- gGCC-GCAGGcGGCUGUGCUGGGCCAu -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 7962 | 0.67 | 0.29525 |
Target: 5'- gCgGGCGUCaCGCCGu--CG-CCCGGg- -3' miRNA: 3'- -GgCCGCAG-GCGGCuguGCuGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 7191 | 0.67 | 0.288206 |
Target: 5'- uCCGGCgGUCagCGCgGGCAgucugcauacCGGCCCGGa- -3' miRNA: 3'- -GGCCG-CAG--GCGgCUGU----------GCUGGGCCau -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 29048 | 0.68 | 0.254963 |
Target: 5'- cCCGuGCGgUCGCCGAgUAUGACCgGGUc -3' miRNA: 3'- -GGC-CGCaGGCGGCU-GUGCUGGgCCAu -5' |
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8073 | 3' | -61.8 | NC_001978.2 | + | 17841 | 0.68 | 0.261351 |
Target: 5'- uCCGGCaaggaCGCUGACgGCGugCCGGg- -3' miRNA: 3'- -GGCCGcag--GCGGCUG-UGCugGGCCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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