Results 1 - 20 of 102 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9032 | 5' | -55.6 | NC_002512.2 | + | 36190 | 0.66 | 0.974583 |
Target: 5'- gACGCGUGcAACCGGccccuaucCGAGgUCCGa -3' miRNA: 3'- -UGUGCGC-UUGGCCcaaa----GCUCgAGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 156425 | 0.67 | 0.948228 |
Target: 5'- gGCAUGCuguccgcGGCCGGGcugggGGGCUCCGg -3' miRNA: 3'- -UGUGCGc------UUGGCCCaaag-CUCGAGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 194020 | 0.67 | 0.939537 |
Target: 5'- aACGCGaUGGACCGGGagUCG-GCgaUCCa -3' miRNA: 3'- -UGUGC-GCUUGGCCCaaAGCuCG--AGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 152598 | 1.09 | 0.005146 |
Target: 5'- gACACGCGAACCGGGUUUCGAGCUCCGg -3' miRNA: 3'- -UGUGCGCUUGGCCCAAAGCUCGAGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 87816 | 0.66 | 0.974331 |
Target: 5'- uGCACccccaGCGAucccccgGCCGGGcgUCGGGC-CCu -3' miRNA: 3'- -UGUG-----CGCU-------UGGCCCaaAGCUCGaGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 164892 | 0.66 | 0.972248 |
Target: 5'- -gGCGCG-ACgGGGUUgaucaagacgcagugCGAGCUCUc -3' miRNA: 3'- ugUGCGCuUGgCCCAAa--------------GCUCGAGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 196407 | 0.66 | 0.969189 |
Target: 5'- gGCGCGgGGACgCGGGgggCG-GCggCCGg -3' miRNA: 3'- -UGUGCgCUUG-GCCCaaaGCuCGa-GGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 96737 | 0.66 | 0.969189 |
Target: 5'- cCGCGgGAACCGGacgagCGAGCggggccccgUCCGa -3' miRNA: 3'- uGUGCgCUUGGCCcaaa-GCUCG---------AGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 219940 | 0.66 | 0.968314 |
Target: 5'- gACGCGCGGGggaggguggaucccCCGGGUcgCGcGCUCa- -3' miRNA: 3'- -UGUGCGCUU--------------GGCCCAaaGCuCGAGgc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 193961 | 0.67 | 0.956048 |
Target: 5'- -gGCGCGAgaggccGCCGG----CGAGCUCCu -3' miRNA: 3'- ugUGCGCU------UGGCCcaaaGCUCGAGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 123521 | 0.66 | 0.966205 |
Target: 5'- aACACcugaCGGGCCGcgaGGUggCGAGCUCgCGg -3' miRNA: 3'- -UGUGc---GCUUGGC---CCAaaGCUCGAG-GC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 104690 | 0.66 | 0.969189 |
Target: 5'- gACACGCGcGACCGGaGUcUCGucCUCUc -3' miRNA: 3'- -UGUGCGC-UUGGCC-CAaAGCucGAGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 150508 | 0.66 | 0.974583 |
Target: 5'- cGCcCGCGggUCGGGg--UGcAGUUCCa -3' miRNA: 3'- -UGuGCGCuuGGCCCaaaGC-UCGAGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 227209 | 0.66 | 0.963024 |
Target: 5'- cGCGCGCGGACCaGGaggCG-GCggaCCGg -3' miRNA: 3'- -UGUGCGCUUGGcCCaaaGCuCGa--GGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 55194 | 0.66 | 0.974583 |
Target: 5'- uUACGUGggUauaaaaaGGGUagugccgUCGAGCUCUGc -3' miRNA: 3'- uGUGCGCuuGg------CCCAa------AGCUCGAGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 221399 | 0.66 | 0.969189 |
Target: 5'- -gGCGCGGGCgGGGaggcggCGGGUcugUCCGa -3' miRNA: 3'- ugUGCGCUUGgCCCaaa---GCUCG---AGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 116171 | 0.66 | 0.963024 |
Target: 5'- cGCugGC--GCCGGG---CGAGUUCCa -3' miRNA: 3'- -UGugCGcuUGGCCCaaaGCUCGAGGc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 227098 | 0.67 | 0.943992 |
Target: 5'- gGC-CGCcGGCCGGG--UCGGGCUCa- -3' miRNA: 3'- -UGuGCGcUUGGCCCaaAGCUCGAGgc -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 209434 | 0.66 | 0.974331 |
Target: 5'- cCGCGCGGaugaccgucaucgGCCGGcugaucUUCG-GCUCCGg -3' miRNA: 3'- uGUGCGCU-------------UGGCCca----AAGCuCGAGGC- -5' |
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9032 | 5' | -55.6 | NC_002512.2 | + | 77551 | 0.66 | 0.971979 |
Target: 5'- uACGuCGCGGACggCGGGUcgcccaCGGGCgUCCGa -3' miRNA: 3'- -UGU-GCGCUUG--GCCCAaa----GCUCG-AGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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