Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9158 | 5' | -48.9 | NC_002512.2 | + | 153920 | 0.66 | 0.9999 |
Target: 5'- aACGGuuCCGUgCAuUCGAgUCGAGAa -3' miRNA: 3'- gUGCCuuGGCAaGU-AGCUgAGCUUUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 155272 | 0.66 | 0.9999 |
Target: 5'- uCGCgGGAGCCGagg--CGGCUCGAGc- -3' miRNA: 3'- -GUG-CCUUGGCaaguaGCUGAGCUUua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 99821 | 0.66 | 0.9999 |
Target: 5'- -uCGGGACCGUccagggacgcgUCAUUGACUuuauugaauaCGGAAUa -3' miRNA: 3'- guGCCUUGGCA-----------AGUAGCUGA----------GCUUUA- -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 123108 | 0.66 | 0.9999 |
Target: 5'- gGCGGAACCGUcccgccgcCGUCGGCggccguccCGggGg -3' miRNA: 3'- gUGCCUUGGCAa-------GUAGCUGa-------GCuuUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 184759 | 0.66 | 0.99987 |
Target: 5'- gGCGGGcggcCCGUggUCGUCGACccCGAGGa -3' miRNA: 3'- gUGCCUu---GGCA--AGUAGCUGa-GCUUUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 228990 | 0.66 | 0.99987 |
Target: 5'- aGCGGAACCGUgg--CGGCgggacuagCGGGAg -3' miRNA: 3'- gUGCCUUGGCAaguaGCUGa-------GCUUUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 12584 | 0.66 | 0.99987 |
Target: 5'- gGCGGAGCCGaUCAUcuccCGGCgcgCGGu-- -3' miRNA: 3'- gUGCCUUGGCaAGUA----GCUGa--GCUuua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 31699 | 0.66 | 0.999833 |
Target: 5'- aCGCGGAcCUGUUCGcCGACgUGGAGg -3' miRNA: 3'- -GUGCCUuGGCAAGUaGCUGaGCUUUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 156267 | 0.66 | 0.999833 |
Target: 5'- uUACGGAACCGgaguugCAgaggCGACUCc---- -3' miRNA: 3'- -GUGCCUUGGCaa----GUa---GCUGAGcuuua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 99325 | 0.66 | 0.999788 |
Target: 5'- uCACGGucCUGUaCGUCGuCUCGGAc- -3' miRNA: 3'- -GUGCCuuGGCAaGUAGCuGAGCUUua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 131547 | 0.66 | 0.999788 |
Target: 5'- aACGG--CCaGUUCGUCGcGCUCGGGAg -3' miRNA: 3'- gUGCCuuGG-CAAGUAGC-UGAGCUUUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 152245 | 0.66 | 0.999788 |
Target: 5'- uGCGGAACgugUCGUCG-CUCGAc-- -3' miRNA: 3'- gUGCCUUGgcaAGUAGCuGAGCUuua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 119349 | 0.66 | 0.999788 |
Target: 5'- gGCGGAaacGCCGgUCAcggCGACUCGc--- -3' miRNA: 3'- gUGCCU---UGGCaAGUa--GCUGAGCuuua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 173298 | 0.66 | 0.999731 |
Target: 5'- gCugGaGGGCCG-UCAUCGGCUCc---- -3' miRNA: 3'- -GugC-CUUGGCaAGUAGCUGAGcuuua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 217801 | 0.66 | 0.999731 |
Target: 5'- gGCGGAucggcgucGCCGUcccgaUCGUCGACggCGGGc- -3' miRNA: 3'- gUGCCU--------UGGCA-----AGUAGCUGa-GCUUua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 124283 | 0.67 | 0.999661 |
Target: 5'- -uCGGAcCCGggCGUgCGGCUCGAc-- -3' miRNA: 3'- guGCCUuGGCaaGUA-GCUGAGCUuua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 152305 | 0.67 | 0.999576 |
Target: 5'- --aGGGACCGcUCGgcgUGGCUCGGAc- -3' miRNA: 3'- gugCCUUGGCaAGUa--GCUGAGCUUua -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 190627 | 0.67 | 0.999576 |
Target: 5'- -uCGGAACUGUcuucgUCAUCGGCgucgucaCGAGAUu -3' miRNA: 3'- guGCCUUGGCA-----AGUAGCUGa------GCUUUA- -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 121352 | 0.67 | 0.999474 |
Target: 5'- gGCGGGGCCGUUgGaCGGCggaGAGGg -3' miRNA: 3'- gUGCCUUGGCAAgUaGCUGag-CUUUa -5' |
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9158 | 5' | -48.9 | NC_002512.2 | + | 77444 | 0.67 | 0.99935 |
Target: 5'- uCACGGAcgACCGagCGUCGAUUCc---- -3' miRNA: 3'- -GUGCCU--UGGCaaGUAGCUGAGcuuua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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