Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1088 | 5' | -54.7 | NC_000929.1 | + | 31117 | 0.73 | 0.241665 |
Target: 5'- aCGGCACccuguccgaacGCCAGCGGGCU---GCCg -3' miRNA: 3'- gGCCGUGa----------CGGUCGUCCGAuuuUGGg -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 30907 | 0.66 | 0.627059 |
Target: 5'- aCGGCcuuucugcaauuACUGCCGucaGGGCUGccuggaauaaAAGCCCc -3' miRNA: 3'- gGCCG------------UGACGGUcg-UCCGAU----------UUUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 30119 | 0.66 | 0.625918 |
Target: 5'- gCCGGUACacGCUGGCAGcguGCUGAuggugugguuuacAGCCUg -3' miRNA: 3'- -GGCCGUGa-CGGUCGUC---CGAUU-------------UUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 30025 | 0.66 | 0.59291 |
Target: 5'- -gGaGCACUGCCGGUucugGGGCgugcGCCg -3' miRNA: 3'- ggC-CGUGACGGUCG----UCCGauuuUGGg -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 28723 | 0.68 | 0.48269 |
Target: 5'- aCCGGCACcagaaaGCCuucgcgugguGCaAGGCUGAcgcggguuuucAGCCCg -3' miRNA: 3'- -GGCCGUGa-----CGGu---------CG-UCCGAUU-----------UUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 28240 | 0.73 | 0.254968 |
Target: 5'- gUGGCugUGCUGGCAgcccGGCUGAAuACCa -3' miRNA: 3'- gGCCGugACGGUCGU----CCGAUUU-UGGg -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 28183 | 0.67 | 0.581585 |
Target: 5'- gCCGG-GCUGCCAGCAcagccacugauGaGCUGGuuCUCg -3' miRNA: 3'- -GGCCgUGACGGUCGU-----------C-CGAUUuuGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 27254 | 0.67 | 0.559079 |
Target: 5'- gCUGGCugUGCCGGUuguGGUggacgGGGcAUCCa -3' miRNA: 3'- -GGCCGugACGGUCGu--CCGa----UUU-UGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 27204 | 0.76 | 0.173114 |
Target: 5'- aCCGGCACaGCCAGCGGGgccagcagcacauCUGcuACCg -3' miRNA: 3'- -GGCCGUGaCGGUCGUCC-------------GAUuuUGGg -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 27100 | 0.71 | 0.347538 |
Target: 5'- aCCGGgcagaaGCUGCCAGCcAGGCacuGACUg -3' miRNA: 3'- -GGCCg-----UGACGGUCG-UCCGauuUUGGg -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 26696 | 0.66 | 0.59291 |
Target: 5'- aCGGcCugUGCCGGUAcGGCUGcacuGGugUCu -3' miRNA: 3'- gGCC-GugACGGUCGU-CCGAU----UUugGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 26012 | 0.69 | 0.452521 |
Target: 5'- cCCGGCgacACUGcCCAGCGuGGCaccagcaaccguACCCa -3' miRNA: 3'- -GGCCG---UGAC-GGUCGU-CCGauuu--------UGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 25931 | 0.68 | 0.490118 |
Target: 5'- aCCGGCuguACUGCCAauuGCgccugcuuuuucauGGGUUGAcGCCUg -3' miRNA: 3'- -GGCCG---UGACGGU---CG--------------UCCGAUUuUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 25659 | 0.67 | 0.559079 |
Target: 5'- cCCGGCagcACUGCCugcaAGCcuGCUcagAAGACCg -3' miRNA: 3'- -GGCCG---UGACGG----UCGucCGA---UUUUGGg -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 25583 | 0.7 | 0.392626 |
Target: 5'- aCCGGUAC-GCCGGguGGggGuAAugCCa -3' miRNA: 3'- -GGCCGUGaCGGUCguCCgaU-UUugGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 25539 | 0.75 | 0.17855 |
Target: 5'- -aGGUAaUGCCAGC-GGCUGugGCCCg -3' miRNA: 3'- ggCCGUgACGGUCGuCCGAUuuUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 25169 | 0.71 | 0.336437 |
Target: 5'- uCUGGC-UUGCCaauaaagcccugagAGCAGGCUGggccguggcaAAACCCu -3' miRNA: 3'- -GGCCGuGACGG--------------UCGUCCGAU----------UUUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 24736 | 0.71 | 0.347538 |
Target: 5'- aCCGuaACcgGUCAGCAGGUUGGcAUCCg -3' miRNA: 3'- -GGCcgUGa-CGGUCGUCCGAUUuUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 24096 | 0.7 | 0.399235 |
Target: 5'- gCUGGCAgUGCCggaaaaagcgggacAGCGGGagagacAAGGCCCa -3' miRNA: 3'- -GGCCGUgACGG--------------UCGUCCga----UUUUGGG- -5' |
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1088 | 5' | -54.7 | NC_000929.1 | + | 24046 | 1.13 | 0.000311 |
Target: 5'- uCCGGCACUGCCAGCAGGCUAAAACCCu -3' miRNA: 3'- -GGCCGUGACGGUCGUCCGAUUUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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