Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12336 | 5' | -54.3 | NC_003324.1 | + | 42015 | 0.68 | 0.633813 |
Target: 5'- aGCGCaUCGGGAACGCgguaccucCGGAuGCgGCGa -3' miRNA: 3'- -CGCG-AGUUCUUGCG--------GCUUuCGgCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 14468 | 0.66 | 0.793369 |
Target: 5'- cGCGCUCGguugcGGcGCGuuGAuuggcagcAGCCGUGg -3' miRNA: 3'- -CGCGAGU-----UCuUGCggCUu-------UCGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 5174 | 0.66 | 0.783494 |
Target: 5'- gGCGCagaucgUCGAucGCGCCGcuuaagcuaucAGGCCGCGUu -3' miRNA: 3'- -CGCG------AGUUcuUGCGGCu----------UUCGGCGCA- -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 26255 | 0.67 | 0.699747 |
Target: 5'- cCGCagCGAGAcCGCCGAcagaaugaugcAGGCCGCc- -3' miRNA: 3'- cGCGa-GUUCUuGCGGCU-----------UUCGGCGca -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 18906 | 0.67 | 0.688858 |
Target: 5'- gGCGUgaUgGAGAcugGCGCCGAccuucGCCGCGc -3' miRNA: 3'- -CGCG--AgUUCU---UGCGGCUuu---CGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 12741 | 0.66 | 0.763279 |
Target: 5'- uGCGUUCGccAG-ACGCCGAAAgGuuGuCGUa -3' miRNA: 3'- -CGCGAGU--UCuUGCGGCUUU-CggC-GCA- -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 51661 | 0.67 | 0.721312 |
Target: 5'- uUGCUCGGGAAUaCCGAAGGCgccuuacccuUGCGa -3' miRNA: 3'- cGCGAGUUCUUGcGGCUUUCG----------GCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 29026 | 0.67 | 0.710568 |
Target: 5'- uUGCUCAAGcAGCGCCaGgcGGCaCGCu- -3' miRNA: 3'- cGCGAGUUC-UUGCGG-CuuUCG-GCGca -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 21220 | 0.68 | 0.666929 |
Target: 5'- uCGCUCAcuGACgagGCCGAGAuGCUGCGc -3' miRNA: 3'- cGCGAGUucUUG---CGGCUUU-CGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 19090 | 0.69 | 0.611711 |
Target: 5'- cGCGUUCGAGGccaGCCGcgugcAGCCGcCGUa -3' miRNA: 3'- -CGCGAGUUCUug-CGGCuu---UCGGC-GCA- -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 17283 | 1.09 | 0.001391 |
Target: 5'- gGCGCUCAAGAACGCCGAAAGCCGCGUc -3' miRNA: 3'- -CGCGAGUUCUUGCGGCUUUCGGCGCA- -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 24147 | 0.67 | 0.736201 |
Target: 5'- aGCGCaccggCGAGAAgguaaagcccgccagUGCCGAgaAGGCCGCc- -3' miRNA: 3'- -CGCGa----GUUCUU---------------GCGGCU--UUCGGCGca -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 47794 | 0.66 | 0.763279 |
Target: 5'- uGCGguuCUCAAGcagcauguCGUCGAggAGGCCGCGa -3' miRNA: 3'- -CGC---GAGUUCuu------GCGGCU--UUCGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 50626 | 0.67 | 0.688858 |
Target: 5'- uGCGCUCGgcgucgagcAGGucaACGCgGGugcuGGCUGCGUg -3' miRNA: 3'- -CGCGAGU---------UCU---UGCGgCUu---UCGGCGCA- -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 26203 | 0.68 | 0.666929 |
Target: 5'- aCGgUCGAGAucagguaGCCGAAGGCC-CGUu -3' miRNA: 3'- cGCgAGUUCUug-----CGGCUUUCGGcGCA- -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 14600 | 0.67 | 0.731967 |
Target: 5'- aGCGCcuugUCGcGGACGCCcu--GCCGCGc -3' miRNA: 3'- -CGCG----AGUuCUUGCGGcuuuCGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 22122 | 0.69 | 0.611711 |
Target: 5'- aUGCUCAAGGucaugucggACGCgCGcAAGGCUGCGc -3' miRNA: 3'- cGCGAGUUCU---------UGCG-GC-UUUCGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 5115 | 0.69 | 0.58968 |
Target: 5'- gGUGUcgaUCGAGAucACGCCGu-AGUCGCGg -3' miRNA: 3'- -CGCG---AGUUCU--UGCGGCuuUCGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 28689 | 0.67 | 0.721312 |
Target: 5'- cGgGCcaCGAGGGCGacagcgggCGGAAGCCGCGa -3' miRNA: 3'- -CgCGa-GUUCUUGCg-------GCUUUCGGCGCa -5' |
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12336 | 5' | -54.3 | NC_003324.1 | + | 31128 | 0.67 | 0.688858 |
Target: 5'- aGCGCgucagCAGGAguugaACGCCGA---CCGCGc -3' miRNA: 3'- -CGCGa----GUUCU-----UGCGGCUuucGGCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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