miRNA: 3'- aUGcGGACGCGA-CGGCGguuCCGUag -5'
miRNA: 3'- auGCGGa--CG-CGACGGCGGuuCCGUag -5'
miRNA: 3'- aUGCGGaCGCGACGGcGGUUCCguag -5'
miRNA: 3'- aUGCGGACGCGACGGcGGuuCCGUag -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12458 | 3' | -60.3 | NC_003324.1 | + | 16318 | 0.66 | 0.379711 |
|
Target: 5'- gGC-CCUGCGCUgGCCGCgucGGCAc- -3' miRNA: 3'- aUGcGGACGCGA-CGGCGguuCCGUag -5' |
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| 12458 | 3' | -60.3 | NC_003324.1 | + | 35098 | 0.66 | 0.379711 |
|
Target: 5'- -cCGCCgccGCuGCUGCUGCUgcGGCAg- -3' miRNA: 3'- auGCGGa--CG-CGACGGCGGuuCCGUag -5' |
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| 12458 | 3' | -60.3 | NC_003324.1 | + | 28579 | 0.66 | 0.388543 |
|
Target: 5'- gGCGCCcGCGUUGCCaUCAAGGa--- -3' miRNA: 3'- aUGCGGaCGCGACGGcGGUUCCguag -5' |
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| 12458 | 3' | -60.3 | NC_003324.1 | + | 52624 | 0.66 | 0.397509 |
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Target: 5'- cAUGCUUGUGCUGCCuCCc-GGCGg- -3' miRNA: 3'- aUGCGGACGCGACGGcGGuuCCGUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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