Results 1 - 20 of 120 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17006 | 5' | -62.5 | NC_004333.2 | + | 30730 | 0.66 | 0.33247 |
Target: 5'- aGCUCG-GCCGuGgCGUCGCCGaGgUACu -3' miRNA: 3'- -UGAGCgCGGUuCgGCAGCGGC-CgGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 28763 | 0.66 | 0.33247 |
Target: 5'- cGC-CGCGUCGA-CCG-CGCgGGUCGCg -3' miRNA: 3'- -UGaGCGCGGUUcGGCaGCGgCCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 17059 | 0.66 | 0.33247 |
Target: 5'- uGCUCGCGguguuCC-AGCCG-CGCCGGa-ACa -3' miRNA: 3'- -UGAGCGC-----GGuUCGGCaGCGGCCggUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 44820 | 0.66 | 0.33247 |
Target: 5'- cGCUCG-GCgAGGacauggaCGaCGCUGGCCGCg -3' miRNA: 3'- -UGAGCgCGgUUCg------GCaGCGGCCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 36303 | 0.66 | 0.33247 |
Target: 5'- uGCUCGCGCCcguuucGGCCGa--CCGGCagACg -3' miRNA: 3'- -UGAGCGCGGu-----UCGGCagcGGCCGg-UG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 39948 | 0.66 | 0.324721 |
Target: 5'- cCUCGCG-CGGGCCGgCGUCauccuGCCGCg -3' miRNA: 3'- uGAGCGCgGUUCGGCaGCGGc----CGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 11289 | 0.66 | 0.324721 |
Target: 5'- uGCcgCGCGCUGuugaacugcGCCGUCGCCuuguugcacuGGUCGCg -3' miRNA: 3'- -UGa-GCGCGGUu--------CGGCAGCGG----------CCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 33944 | 0.66 | 0.324721 |
Target: 5'- gGCgCGCGCgCAcGCCGUCGCaugGGUCu- -3' miRNA: 3'- -UGaGCGCG-GUuCGGCAGCGg--CCGGug -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 17468 | 0.66 | 0.324721 |
Target: 5'- aGCUUGCcagcuuCCAGgacgaaguuGCCGcCGCCGGCgGCg -3' miRNA: 3'- -UGAGCGc-----GGUU---------CGGCaGCGGCCGgUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 4836 | 0.66 | 0.317106 |
Target: 5'- cGCUCGUucuGCCAgauGGCCGUCaugauuuCCGGCguCg -3' miRNA: 3'- -UGAGCG---CGGU---UCGGCAGc------GGCCGguG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 36354 | 0.66 | 0.317106 |
Target: 5'- aGCUggaGCGC---GCCGUCGCgcugaUGGCCGCg -3' miRNA: 3'- -UGAg--CGCGguuCGGCAGCG-----GCCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 19437 | 0.66 | 0.309628 |
Target: 5'- cGCUCGCGCa-----GUUGCUGGCCGa -3' miRNA: 3'- -UGAGCGCGguucggCAGCGGCCGGUg -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 35790 | 0.66 | 0.309628 |
Target: 5'- cACUggCGCGCCGGGCgGggCGCgGGCgGg -3' miRNA: 3'- -UGA--GCGCGGUUCGgCa-GCGgCCGgUg -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 22656 | 0.66 | 0.309628 |
Target: 5'- aGCggCGCGuaCCAAGaCG-CGCuCGGCCGCa -3' miRNA: 3'- -UGa-GCGC--GGUUCgGCaGCG-GCCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 36305 | 0.66 | 0.309628 |
Target: 5'- --aCGCGCCGcccgAGuaGUCGCCGGgCGu -3' miRNA: 3'- ugaGCGCGGU----UCggCAGCGGCCgGUg -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 1120 | 0.66 | 0.309628 |
Target: 5'- --gCGCGCgAAGUCGgUGCCGuGCUGCg -3' miRNA: 3'- ugaGCGCGgUUCGGCaGCGGC-CGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 22746 | 0.66 | 0.308887 |
Target: 5'- gGCaCGC-CCGgcgugacGGCCGcCGCCGGCaCGCu -3' miRNA: 3'- -UGaGCGcGGU-------UCGGCaGCGGCCG-GUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 38138 | 0.66 | 0.302284 |
Target: 5'- cUUCGCGUuCGAcGCCGaC-CCGGCCGCc -3' miRNA: 3'- uGAGCGCG-GUU-CGGCaGcGGCCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 19152 | 0.66 | 0.302284 |
Target: 5'- uGCUCGCGCUGguuaucgugcuAGCCGcgcgcgaugCGCacgccgacccggCGGCCGCg -3' miRNA: 3'- -UGAGCGCGGU-----------UCGGCa--------GCG------------GCCGGUG- -5' |
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17006 | 5' | -62.5 | NC_004333.2 | + | 23057 | 0.66 | 0.302284 |
Target: 5'- aACgugCGCGCC--GCCGugcUCGCCGuGCC-Cg -3' miRNA: 3'- -UGa--GCGCGGuuCGGC---AGCGGC-CGGuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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