Results 1 - 20 of 81 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26270 | 3' | -56.1 | NC_005345.2 | + | 15049 | 0.66 | 0.668032 |
Target: 5'- gGCGAuguCGCCgucgcgGGC-GCCGG-CCUGCa -3' miRNA: 3'- -UGCU---GUGGaaa---CUGuCGGCCaGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 12972 | 0.66 | 0.668032 |
Target: 5'- -gGACACCggcGACcaacgcugcacGCCGGaCCCGCc -3' miRNA: 3'- ugCUGUGGaaaCUGu----------CGGCCaGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 26738 | 0.66 | 0.668032 |
Target: 5'- gUGACAgCgaUGAacCGGCCcGUCCCGUa -3' miRNA: 3'- uGCUGUgGaaACU--GUCGGcCAGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 24816 | 0.66 | 0.668032 |
Target: 5'- gGCGACcggaucguGCUcgggUUcGACGGCucgcgcaagCGGUCCCGCg -3' miRNA: 3'- -UGCUG--------UGGa---AA-CUGUCG---------GCCAGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 29763 | 0.66 | 0.668032 |
Target: 5'- cGCGACGCUgcucGGCAcgaccGCCGaGUCCCu- -3' miRNA: 3'- -UGCUGUGGaaa-CUGU-----CGGC-CAGGGcg -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 37678 | 0.66 | 0.666939 |
Target: 5'- cGCGGCccugaugucgucgGCCgugGGCcGCCaGUCCUGCg -3' miRNA: 3'- -UGCUG-------------UGGaaaCUGuCGGcCAGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 44669 | 0.66 | 0.666938 |
Target: 5'- cACGGCucgucgaGCCg--GACAcggccGCCGGgccCCCGCc -3' miRNA: 3'- -UGCUG-------UGGaaaCUGU-----CGGCCa--GGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 48968 | 0.66 | 0.66475 |
Target: 5'- aGCGGCAUgCgucUGACGcguguggggagcacGCCGG-CCCGCu -3' miRNA: 3'- -UGCUGUG-Gaa-ACUGU--------------CGGCCaGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 39458 | 0.66 | 0.657083 |
Target: 5'- gGCGGCccgcugaucgGCCU---GCAGCCGGcggcgCUCGCg -3' miRNA: 3'- -UGCUG----------UGGAaacUGUCGGCCa----GGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 5919 | 0.66 | 0.657083 |
Target: 5'- uGCGG-GCCac-GACAGCgGGUCgCCGUg -3' miRNA: 3'- -UGCUgUGGaaaCUGUCGgCCAG-GGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 22367 | 0.66 | 0.657083 |
Target: 5'- cGCGGCGCCggccacGAUccGCCGcaCCCGCg -3' miRNA: 3'- -UGCUGUGGaaa---CUGu-CGGCcaGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 32694 | 0.66 | 0.653793 |
Target: 5'- uCGACACCgaggggGAacacaucggcgaacCAGCCGaGUCCCucgGCg -3' miRNA: 3'- uGCUGUGGaaa---CU--------------GUCGGC-CAGGG---CG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 34456 | 0.66 | 0.646111 |
Target: 5'- cGCcACACCacggGcACGGCCGGcCCgGCa -3' miRNA: 3'- -UGcUGUGGaaa-C-UGUCGGCCaGGgCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 414 | 0.66 | 0.646111 |
Target: 5'- cGCGGcCACCacuccauGCGGCCGGugUCCCGg -3' miRNA: 3'- -UGCU-GUGGaaac---UGUCGGCC--AGGGCg -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 32789 | 0.66 | 0.646111 |
Target: 5'- gGCGACGCCgc-GGCGauGCuCGGcgcgUUCCGCg -3' miRNA: 3'- -UGCUGUGGaaaCUGU--CG-GCC----AGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 9345 | 0.66 | 0.645013 |
Target: 5'- uGCGACGCaugguggCUgcgGGCGGCCGGgCUCGa -3' miRNA: 3'- -UGCUGUG-------GAaa-CUGUCGGCCaGGGCg -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 13215 | 0.66 | 0.635126 |
Target: 5'- gACGACGCCgccGACcuucuucGCCGcGUCCC-Cg -3' miRNA: 3'- -UGCUGUGGaaaCUGu------CGGC-CAGGGcG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 14507 | 0.66 | 0.635126 |
Target: 5'- cGCGACGCCgagcGGCAGCagccacGcCCCGUa -3' miRNA: 3'- -UGCUGUGGaaa-CUGUCGgc----CaGGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 16966 | 0.66 | 0.635126 |
Target: 5'- uCGGCACCU----CGGCCGGcUgCCGUg -3' miRNA: 3'- uGCUGUGGAaacuGUCGGCC-AgGGCG- -5' |
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26270 | 3' | -56.1 | NC_005345.2 | + | 34086 | 0.66 | 0.635126 |
Target: 5'- -aGGCGCUgca---GGCCGGcgCCCGCg -3' miRNA: 3'- ugCUGUGGaaacugUCGGCCa-GGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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