Results 1 - 20 of 73 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28006 | 3' | -59.9 | NC_005887.1 | + | 6049 | 0.66 | 0.428947 |
Target: 5'- gGUGCUGgu-GGCAgCggCGCGGCAc -3' miRNA: 3'- gCGCGGCaagCCGUgGaaGCGCCGUc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 10224 | 0.66 | 0.428947 |
Target: 5'- cCGCGCCGgaCGGCGCguCUgaugaccUGUGGguGa -3' miRNA: 3'- -GCGCGGCaaGCCGUG--GAa------GCGCCguC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 2432 | 0.66 | 0.428947 |
Target: 5'- aCGCGCC---CGGCucgAUCUgCGCGGCAc -3' miRNA: 3'- -GCGCGGcaaGCCG---UGGAaGCGCCGUc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 4920 | 0.66 | 0.428947 |
Target: 5'- aGCGCCGcgcuccacUCcGCACCUggcgUGCaGGCAGu -3' miRNA: 3'- gCGCGGCa-------AGcCGUGGAa---GCG-CCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 36589 | 0.66 | 0.419582 |
Target: 5'- uCGCgGCCa----GCGCCUUCGCGGCc- -3' miRNA: 3'- -GCG-CGGcaagcCGUGGAAGCGCCGuc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 23259 | 0.66 | 0.419582 |
Target: 5'- gCGCGCaaaa-GGCGCagcUCGUGGCGGu -3' miRNA: 3'- -GCGCGgcaagCCGUGga-AGCGCCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 41573 | 0.66 | 0.419582 |
Target: 5'- aGCGCCGagacguuccugUCGGCcagcggcgGCCcaggUGCGGCGGc -3' miRNA: 3'- gCGCGGCa----------AGCCG--------UGGaa--GCGCCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 12063 | 0.66 | 0.419582 |
Target: 5'- cCGCGCUGcagcaggUCGGCACg-UCGCucGGuCAGg -3' miRNA: 3'- -GCGCGGCa------AGCCGUGgaAGCG--CC-GUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 27838 | 0.66 | 0.419582 |
Target: 5'- cCGCGCCugcaagCGGacCACCaagcacCGCGGCGGg -3' miRNA: 3'- -GCGCGGcaa---GCC--GUGGaa----GCGCCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 28916 | 0.66 | 0.410343 |
Target: 5'- aCGUGCUGagCGGUgcGCCgcucgUCGCaGCAGc -3' miRNA: 3'- -GCGCGGCaaGCCG--UGGa----AGCGcCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 25497 | 0.66 | 0.410343 |
Target: 5'- cCGCGCCG--CGGUACaCcUCGCGGa-- -3' miRNA: 3'- -GCGCGGCaaGCCGUG-GaAGCGCCguc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 15273 | 0.66 | 0.407596 |
Target: 5'- aGCGCagcaaGcgCGGCACCgagUUcgugaagaagucggGCGGCAGc -3' miRNA: 3'- gCGCGg----CaaGCCGUGGa--AG--------------CGCCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 23935 | 0.66 | 0.40123 |
Target: 5'- aGCGUCGUgcugacgaCGGCGCC---GCGGCGu -3' miRNA: 3'- gCGCGGCAa-------GCCGUGGaagCGCCGUc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 7453 | 0.66 | 0.40123 |
Target: 5'- gCGCGUCGUgaUCGGCauGCCgUUCGCGaacgaGCGc -3' miRNA: 3'- -GCGCGGCA--AGCCG--UGG-AAGCGC-----CGUc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 16027 | 0.66 | 0.392248 |
Target: 5'- aGCGCCGgccaCGGCcugaACCUgcaggacgGCGGCAa -3' miRNA: 3'- gCGCGGCaa--GCCG----UGGAag------CGCCGUc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 11884 | 0.66 | 0.392248 |
Target: 5'- -uCGUCGUUCGGCAgcgccggcaaCUUCaCGGCGGg -3' miRNA: 3'- gcGCGGCAAGCCGUg---------GAAGcGCCGUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 27786 | 0.66 | 0.383397 |
Target: 5'- aGCGCCaugacgaCGGCGCCgaCGCaGGCGa -3' miRNA: 3'- gCGCGGcaa----GCCGUGGaaGCG-CCGUc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 26522 | 0.66 | 0.383397 |
Target: 5'- uCGCGCCGcgcaagCGcuGCACCUgcgccUCGUGGuCAGu -3' miRNA: 3'- -GCGCGGCaa----GC--CGUGGA-----AGCGCC-GUC- -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 41498 | 0.66 | 0.383397 |
Target: 5'- aCGCGUCcacgGGCACCUuggcuuucuUCGCGGCc- -3' miRNA: 3'- -GCGCGGcaagCCGUGGA---------AGCGCCGuc -5' |
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28006 | 3' | -59.9 | NC_005887.1 | + | 37596 | 0.66 | 0.383397 |
Target: 5'- cCGCGCuCG-UCGcaaaaGCACCgccgguauugcUCGCGGCGGu -3' miRNA: 3'- -GCGCG-GCaAGC-----CGUGGa----------AGCGCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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