Results 1 - 20 of 112 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5548 | 5' | -61.7 | NC_001806.1 | + | 106251 | 0.66 | 0.70408 |
Target: 5'- uGGGuAACguuuucggccgUCCCUga-CCCCGAgGCCCu -3' miRNA: 3'- uCCC-UUG-----------AGGGAgagGGGGCUgCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 132705 | 0.66 | 0.70408 |
Target: 5'- uGGGGGCcagggucgUCCUC-CCCCCGcgaGCugGCCCg -3' miRNA: 3'- uCCCUUGa-------GGGAGaGGGGGC---UG--CGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 68465 | 0.66 | 0.70408 |
Target: 5'- uGGuGGcuCUCCC-CgaggCCCCCGACcagGCCUu -3' miRNA: 3'- -UC-CCuuGAGGGaGa---GGGGGCUG---CGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 107816 | 0.66 | 0.70408 |
Target: 5'- gGGGGAucggcuacaaGCUCaCCggCgucgaCgUCCGACGCCCa -3' miRNA: 3'- -UCCCU----------UGAG-GGa-Ga----GgGGGCUGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 6979 | 0.66 | 0.70408 |
Target: 5'- cGGaGGGugUUCC---CCCCCGugGCUCu -3' miRNA: 3'- -UC-CCUugAGGGagaGGGGGCugCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 56687 | 0.66 | 0.70408 |
Target: 5'- gGGGGGACgggggCCCgggaaCCCCGGguCGCUCu -3' miRNA: 3'- -UCCCUUGa----GGGagag-GGGGCU--GCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 70495 | 0.66 | 0.70408 |
Target: 5'- cGGGGACacgCCCga-CCgCCUGGgGCCCu -3' miRNA: 3'- uCCCUUGa--GGGagaGG-GGGCUgCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 55251 | 0.66 | 0.694489 |
Target: 5'- gAGGGucGGCgCCCgggCcggCCCCCucCGCCCa -3' miRNA: 3'- -UCCC--UUGaGGGa--Ga--GGGGGcuGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 62803 | 0.66 | 0.694489 |
Target: 5'- aGGGGGGCaaagCCCUCg---CUGACGUCCg -3' miRNA: 3'- -UCCCUUGa---GGGAGagggGGCUGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 22724 | 0.66 | 0.694489 |
Target: 5'- cGGGGGGCga-Cga--CCCCGACGCCg -3' miRNA: 3'- -UCCCUUGaggGagagGGGGCUGCGGg -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 23526 | 0.66 | 0.693527 |
Target: 5'- gAGGGccccGACcCCCUgggcggcuggcggCggcagCCCCCGGgGCCCa -3' miRNA: 3'- -UCCC----UUGaGGGA-------------Ga----GGGGGCUgCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 97596 | 0.66 | 0.68485 |
Target: 5'- uGGGGGACUgCaUCggcaaggacgCCCgCGACGCCa -3' miRNA: 3'- -UCCCUUGAgGgAGa---------GGGgGCUGCGGg -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 102960 | 0.66 | 0.68485 |
Target: 5'- cGGGGGCgCC----CCCCCGuCGCCCc -3' miRNA: 3'- uCCCUUGaGGgagaGGGGGCuGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 127953 | 0.66 | 0.683884 |
Target: 5'- gAGGGcgacgcgGACUCCCgUCUgagCCCCCGgccacuGCGUCUc -3' miRNA: 3'- -UCCC-------UUGAGGG-AGA---GGGGGC------UGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 64808 | 0.66 | 0.680983 |
Target: 5'- aGGGGGACgggguaggccgugCCCgUUCCCagaCGugGCCg -3' miRNA: 3'- -UCCCUUGa------------GGGaGAGGGg--GCugCGGg -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 54120 | 0.66 | 0.679047 |
Target: 5'- gGGGGGugUCCCggggcccaggggCCgguaggcguguUCCGAUGCCCg -3' miRNA: 3'- -UCCCUugAGGGaga---------GG-----------GGGCUGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 20150 | 0.66 | 0.675172 |
Target: 5'- -cGGAGagaCCCgC-CCCCCGcCGCCCg -3' miRNA: 3'- ucCCUUga-GGGaGaGGGGGCuGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 150109 | 0.66 | 0.675172 |
Target: 5'- cGGGGcGGC-CCCg-UCCccggggaccaaCCCGGCGCCCc -3' miRNA: 3'- -UCCC-UUGaGGGagAGG-----------GGGCUGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 101552 | 0.66 | 0.674202 |
Target: 5'- -cGGAGgcucuuguuuuuuCUCCCUaaugccccCUCCCCCcuCGCCCa -3' miRNA: 3'- ucCCUU-------------GAGGGA--------GAGGGGGcuGCGGG- -5' |
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5548 | 5' | -61.7 | NC_001806.1 | + | 83328 | 0.66 | 0.674202 |
Target: 5'- uGGGGAgggGCUuuuggacaccgggCCCUUUUCCgCGGCGgCCa -3' miRNA: 3'- -UCCCU---UGA-------------GGGAGAGGGgGCUGCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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