Results 1 - 20 of 182 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6486 | 5' | -60.5 | NC_001847.1 | + | 100 | 0.66 | 0.720985 |
Target: 5'- uCCgCCCCUgggUCCGGCgCCCCGCg---- -3' miRNA: 3'- -GGgGGGGG---GGGUUGaGGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 193 | 0.73 | 0.32878 |
Target: 5'- gCCCCCgCCCCC-GC-CCCCGCc---- -3' miRNA: 3'- -GGGGGgGGGGGuUGaGGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 237 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 265 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 293 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 321 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 349 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 377 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 405 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 728 | 0.73 | 0.321598 |
Target: 5'- gCCCCCUCCCUccCUUCCCGCg---- -3' miRNA: 3'- gGGGGGGGGGGuuGAGGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 1328 | 0.67 | 0.642273 |
Target: 5'- gCUCCCCgagCCCCAGCgccgcgCCCUGCg---- -3' miRNA: 3'- gGGGGGG---GGGGUUGa-----GGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 2412 | 0.66 | 0.701574 |
Target: 5'- uCgUCCUCCCCCGagggGCcCCCCGCg---- -3' miRNA: 3'- -GgGGGGGGGGGU----UGaGGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 2648 | 0.66 | 0.720985 |
Target: 5'- uUCgUCCUCCCCGucCUCCCCGUc---- -3' miRNA: 3'- -GGgGGGGGGGGUu-GAGGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 4254 | 0.77 | 0.183439 |
Target: 5'- gCCCCCCgCCUCAG-UCCCCGCUUc-- -3' miRNA: 3'- -GGGGGGgGGGGUUgAGGGGCGAAaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 4940 | 0.66 | 0.701574 |
Target: 5'- gCgCCCCCCCgAGCaaCCUCGCUa--- -3' miRNA: 3'- gGgGGGGGGGgUUGa-GGGGCGAaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 5141 | 0.75 | 0.227827 |
Target: 5'- uUCCCCCCCCCCAcCcCCaCCGCgcgUGc -3' miRNA: 3'- -GGGGGGGGGGGUuGaGG-GGCGaaaAC- -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 5874 | 0.68 | 0.582681 |
Target: 5'- uUCCCCUCCCCA--UUCCCGCc---- -3' miRNA: 3'- gGGGGGGGGGGUugAGGGGCGaaaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 5980 | 0.71 | 0.42399 |
Target: 5'- uCCCCUUUCCCCuuCuUCCCUGCUUg-- -3' miRNA: 3'- -GGGGGGGGGGGuuG-AGGGGCGAAaac -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 6012 | 0.66 | 0.720985 |
Target: 5'- gCCgCCCCUCaCCCuuCUCCCuuuCGCgaUUGg -3' miRNA: 3'- -GG-GGGGGG-GGGuuGAGGG---GCGaaAAC- -5' |
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6486 | 5' | -60.5 | NC_001847.1 | + | 6108 | 0.86 | 0.043542 |
Target: 5'- aCCCCCUCCCCAuugggACUCCCCGCUUc-- -3' miRNA: 3'- gGGGGGGGGGGU-----UGAGGGGCGAAaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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