Results 1 - 20 of 379 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8984 | 5' | -62.9 | NC_002512.2 | + | 226737 | 0.65 | 0.720211 |
Target: 5'- gACGAcGGCgggaccgggcuccgGCCGGACGaCGCGGagGGCg -3' miRNA: 3'- aUGCU-CCG--------------CGGCCUGUcGCGCCg-CUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 133989 | 0.65 | 0.719283 |
Target: 5'- aGCuGAGGaCGauccaccacaucccCCGGACGaagaucuGCGCGGUGGCg -3' miRNA: 3'- aUG-CUCC-GC--------------GGCCUGU-------CGCGCCGCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 34924 | 0.66 | 0.7137 |
Target: 5'- gACGAGaCGaCGG-CGGgguCGCGGCGACg -3' miRNA: 3'- aUGCUCcGCgGCCuGUC---GCGCCGCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 198018 | 0.66 | 0.7137 |
Target: 5'- gACgGGGGCGagGGAC-GCGCGcGCGAg -3' miRNA: 3'- aUG-CUCCGCggCCUGuCGCGC-CGCUg -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 75431 | 0.66 | 0.7137 |
Target: 5'- gGCGGGaCGuCCGGAuCGGgGCGG-GACg -3' miRNA: 3'- aUGCUCcGC-GGCCU-GUCgCGCCgCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 101776 | 0.66 | 0.7137 |
Target: 5'- gGCGAgacGGCGCCGucCu-CGCGGCGGa -3' miRNA: 3'- aUGCU---CCGCGGCcuGucGCGCCGCUg -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 220304 | 0.66 | 0.7137 |
Target: 5'- -cCGAGcCGCCGGGgGGC-CGGgGGCc -3' miRNA: 3'- auGCUCcGCGGCCUgUCGcGCCgCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 165397 | 0.66 | 0.7137 |
Target: 5'- -uCGGGGCGCUcaugguccaGGAgGGC-CGGCGGg -3' miRNA: 3'- auGCUCCGCGG---------CCUgUCGcGCCGCUg -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 208888 | 0.66 | 0.7137 |
Target: 5'- cGgGAGGgGCCGGGaauccccGCGCGGgaGGCu -3' miRNA: 3'- aUgCUCCgCGGCCUgu-----CGCGCCg-CUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 71013 | 0.66 | 0.7137 |
Target: 5'- gACGGGGgGCCGccgcCGGC-CGGuCGACg -3' miRNA: 3'- aUGCUCCgCGGCcu--GUCGcGCC-GCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 149418 | 0.66 | 0.712767 |
Target: 5'- cGCGAGGgGaCCGcGGCgggggcgGGCGCGGgGGg -3' miRNA: 3'- aUGCUCCgC-GGC-CUG-------UCGCGCCgCUg -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 103670 | 0.66 | 0.712767 |
Target: 5'- cACGA-GCGCCucgacgucgcagaGGGCGGCggacGCGGCGGg -3' miRNA: 3'- aUGCUcCGCGG-------------CCUGUCG----CGCCGCUg -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 217556 | 0.66 | 0.7109 |
Target: 5'- --aGAGacCGCUGGAagugguucugaaacUAGCGCGGUGACu -3' miRNA: 3'- augCUCc-GCGGCCU--------------GUCGCGCCGCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 219026 | 0.66 | 0.708095 |
Target: 5'- cUGCGAcGUGCUGGucuacgucgucggccGCGGCGCGG-GGCu -3' miRNA: 3'- -AUGCUcCGCGGCC---------------UGUCGCGCCgCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 85563 | 0.66 | 0.704348 |
Target: 5'- gAgGAGGCGCgGGA--GCGgGaGCGGCc -3' miRNA: 3'- aUgCUCCGCGgCCUguCGCgC-CGCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 128777 | 0.66 | 0.704348 |
Target: 5'- gACGAGGCGCUgcucacccucuGGGucucCGGCGUGGUccgcgGGCg -3' miRNA: 3'- aUGCUCCGCGG-----------CCU----GUCGCGCCG-----CUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 8790 | 0.66 | 0.704348 |
Target: 5'- gAgGAGGCGCCGGAgGGCGUc----- -3' miRNA: 3'- aUgCUCCGCGGCCUgUCGCGccgcug -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 139013 | 0.66 | 0.704348 |
Target: 5'- aACaGGGgGuCCGacGGCGGCGCGGaCGGCu -3' miRNA: 3'- aUGcUCCgC-GGC--CUGUCGCGCC-GCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 11455 | 0.66 | 0.704348 |
Target: 5'- gACGGGG-GCCGGGgaaGGgGCGGaGACc -3' miRNA: 3'- aUGCUCCgCGGCCUg--UCgCGCCgCUG- -5' |
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8984 | 5' | -62.9 | NC_002512.2 | + | 89670 | 0.66 | 0.704348 |
Target: 5'- gACGcGGUGCgaaGGCGGCGgGGCGAg -3' miRNA: 3'- aUGCuCCGCGgc-CUGUCGCgCCGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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